Clone:
REAL510
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC, MICS, IHC, IF
Alternative names:
CR, CROM, DAF, TC

Extended validation for CD55 (DAF) Antibody, anti-human,
REAlease
®

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD55 (DAF) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD55 (DAF)-PE (REAL510, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD55 (DAF) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD55 (DAF)-PE (REAL510, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD55 (DAF) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD55 (DAF)-PE (REAL510, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD55 knockout cells. Wildtype (red) and knockout cells (blue) were stained with CD55-PE, clone (REAL510). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD55 knockout cells. Wildtype (red) and knockout cells (blue) were stained with CD55-PE, clone (REAL510). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD55 (DAF) Antibody, anti-human,
REAlease
®

Overview

Clone REAL510 is an antibody fragment derived from the full CD55 (DAF) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL510 recognizes the CD55 antigen, a 70 kDa glycosylphosphatidylinositol-anchored single chain glycoprotein, which is also known as complement decay-accelerating factor (DAF). CD55 is broadly distributed among hematopoietic and non-hematopoietic cells. It is expressed on the plasma membranes of all cell types that are in intimate contact with serum and on the surfaces of epithelial cells lining the extracellular compartments. Variants of the molecule are present in body fluids and in extracellular matrix. CD55 plays multiple physiologic roles including tissue protection from the cytotoxic complement injury, anti-inflammatory function owing to its anti-adherence properties which enhance transmigration of monocytes and macrophages and reduce tissue injury. CD55 plays an essential role during pregnancy and is involved in the protection of the semiallogeneic fetus and in preventing uncontrolled infiltration by white cells. It is exploited in multiple pathologic conditions by pathogens and viruses in chronic tissue infection processes.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

CR, CROM, DAF, TC

Detailed product information

Technical specifications

CloneREAL510
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD55 (DAF)
Alternative names of antigenCR, CROM, DAF, TC
Distribution of antigenB cells, T cells, monocytes, NK cells, epithelial cells
RRIDAB_2819669, AB_2877049, AB_2877050, AB_2819670

Resources for CD55 (DAF) Antibody, anti-human,
REAlease
®

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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