Clone:
REAL144
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC, MICS, IHC, IF
Alternative names:
MOX44, TSPAN25

Extended validation for CD53 Antibody, anti-human,
REAlease
®

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
View details
Overlay histogram showing flow cytometric analysis of CD53 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD53-APC, clone (REAL144). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD53 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD53-APC, clone (REAL144). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD53 Antibody, anti-human,
REAlease
®

Overview

Clone REAL144 is an antibody fragment derived from the full CD53 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL144 recognizes the CD53 antigen, a 35–42 kDa type II transmembrane glycoprotein which is also known as tetraspanin-25. CD53 is expressed on all leukocytes, including plasma cells, but not on platelets, erythrocytes, and non-hematopoietic tissues. CD53 mediates oxidative burst in monocytes and has been shown to interact with a number of proteins including interleukin 4 (IL-4), CD20, CD2, CD9, IL-2, VLA-4, and CD4. Cross-linking of CD53 on monocytes induces a strong increase in intracellular calcium ions, whereas a modest increase is seen in B lymphocytes. In resting B cells, the molecule transmits a co-stimulatory signal and promotes activation through the Ig receptor.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

MOX44, TSPAN25

Detailed product information

Technical specifications

CloneREAL144
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD53
Alternative names of antigenMOX44, TSPAN25
Distribution of antigenleukocytes
RRIDAB_2751080, AB_2784250, AB_2751099

References for CD53 Antibody, anti-human,
REAlease
®

Publications

  1. Angelisova, P. et al. (1990) The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens. Immunogenetics 32(4): 281-285
  2. Maecker, H. T, et al. (1997) The tetraspanin superfamily: molecular facilitators. FASEB J. 11: 428-442
  3. Olweus, J. et al. (1993) CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J. Immunol. 151: 707-716

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REAlease
®

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