Type of antibody:
Primary antibodies, Recombinant antibodies
recombinant human IgG1
Alternative names:
Itga2, gpIa, VLA-2α, VLA-2alpha, DX5

Extended validation for CD49b Antibody, anti-mouse, REAfinity™


Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA541
Cells were incubated with an excess of purified unconjugated CD49b (REA541) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD49b Antibody, anti-mouse, REAfinity™


Clone REA541 recognizes the mouse CD49b antigen, a single-pass type I membrane protein also known as integrin alpha-2 (ITGA2). CD49b together with CD29 (ITGB1) forms the adhesion molecule very late antigen-2 (VLA-2), which binds mainly to collagens I, II, and XI. VLA-2 is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation, and organization of newly synthesized extracellular matrix. It is also a receptor for laminins, collagen C-propeptides, and E-cadherin. Mice homozygous for a null mutation in the CD49b gene die very early in embryogenesis. CD49b is expressed on the vast majority of mouse NK cells and on a subset of NKT cells. It is not as mouse strain-restricted as other NK cell markers, for example, NK1.1, and is expressed by most common inbred mouse strains. Bone marrow (BM) resident memory T helper (Tʜ) cells also express CD49b and the homing of adoptively transferred BM memory Tʜ cells to BM can be efficiently blocked by antibodies to CD49b. This points to a decisive role of CD49b in the trafficking of memory Th cell precursors to the BM niches for memory Tʜ cells.
Additional information: Clone REA541 displays negligible binding to Fc receptors.

Alternative names

Itga2, gpIa, VLA-2α, VLA-2alpha, DX5

Detailed product information

Technical specifications

Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Alternative names of antigenItga2, gpIa, VLA-2α, VLA-2alpha, DX5
Molecular mass of antigen [kDa]126
Distribution of antigenNK cells, NKT cells, T helper cells
Entrez Gene ID16398
RRIDAB_2752210, AB_2784229, AB_2784228, AB_2784230, AB_2658473, AB_2658474, AB_2658477, AB_2658478, AB_2905058, AB_2921818, AB_2922145, AB_2752212

Resources for CD49b Antibody, anti-mouse, REAfinity™


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References for CD49b Antibody, anti-mouse, REAfinity™


  1. Gagliani, N. et al. (2013) Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells. Nat Med 19(6): 739-746
  2. Hanazawa, A. et al. (2013) CD49b/CD69-dependent generation of resting T helper cell memory. Front Immunol 4: 183
  3. Edelman, J. M. et al. (1994) The mouse VLA-2 homologue supports collagen and laminin adhesion but not virus binding. Cell Adhes. Commun. 2(2): 131-143

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