Clone:
REA426
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Blast-1, BCM1, BLAST, MEM-102, SLAMF2, hCD48, mCD48, TCT.1

Extended validation for CD48 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA426
156-4H9++
BJ40-
TÜ145++
MEM-102+
J4.57++
Cells were incubated with an excess of purified unconjugated CD48 (REA426) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD48. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD48 antibodies and plotted against the side scatter. As a control, CD48 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD48. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD48 antibodies and plotted against the side scatter. As a control, CD48 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD48. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD48 antibodies and plotted against the side scatter. As a control, CD48 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD48. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD48 antibodies and plotted against the side scatter. As a control, CD48 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD48. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD48 antibodies and plotted against the side scatter. As a control, CD48 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD48. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD48 antibodies and plotted against the side scatter. As a control, CD48 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD48. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD48 antibodies and plotted against the side scatter. As a control, CD48 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD48 (REA426). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD48 (REA426). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD48 (REA426). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD48 Antibody, anti-human, REAfinity™

Overview

Clone REA426 recognizes the human CD48 antigen, a glycosyl-phosphatidyl-inositol (GPI)-anchored cell-surface protein of the CD2 family of molecules, which is also known as B-lymphocyte activation marker BLAST-1, BCM1, MEM-102, or SLAM family member 2 (SLAMF2). CD48 is expressed on various hematopoietic cells and its expression is regulated by viral and bacterial products and immune-associated proteins. CD48 binds CD2 and other molecules, yet its high-affinity ligand in both mouse and human systems is CD244 (2B4). Despite its lack of an intracellular domain, stimulation of CD48 induces rearrangement of signaling factors in lipid rafts, Lck-kinase activity, and tyrosine phosphorylation. As an adhesion and co-stimulatory molecule, CD48 induces numerous effects in B and T lymphocytes, natural killer cells, mast cells, and eosinophils. Some of these depend upon cell-cell interactions via CD244-CD48 binding.
Additional information: Clone REA426 displays negligible binding to Fc receptors.

Alternative names

Blast-1, BCM1, BLAST, MEM-102, SLAMF2, hCD48, mCD48, TCT.1

Detailed product information

Technical specifications

CloneREA426
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD48
Alternative names of antigenBlast-1, BCM1, BLAST, MEM-102, SLAMF2, hCD48, mCD48, TCT.1
Molecular mass of antigen [kDa]22
Distribution of antigenleukocytes
Entrez Gene ID962
RRIDAB_2819491, AB_2819407, AB_2819406, AB_2876948, AB_2876949, AB_2658415, AB_2658416, AB_2658417, AB_2658418, AB_2658421, AB_2658422, AB_2658425, AB_2658426, AB_2819499

References for CD48 Antibody, anti-human, REAfinity™

Publications

  1. Tarakhovsky, A. et al. (1995) A role for CD5 in TCR-mediated signal transduction and thymocyte selection. Science 269(5223): 535-537
  2. Efanov, A. et al. (2010)
    CD5
    +
    CD23
    +
    leukemic cell populations in TCL1 transgenic mice show significantly increased proliferation and Akt phosphorylation.
    Leukemia 24(5): 970-975
  3. Lundy, S. K. et al. (2014)
    Characterization and activity of Fas ligand producing CD5
    +
    B cells.
    Methods Mol. Biol. 1190: 81-102

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