Clone:
REAL250
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
MER6, Integrin-associated protein (IAP), Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3

Specifications for CD47 Antibody, anti-human, REAdye_lease™

Overview

Clone REAL250 is an antibody fragment derived from the full CD47 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL250 recognizes CD47, a heavily glycosylated protein with an extracellular IgV domain, five transmembrane domains, and a cytoplasmic tail, which exists in four different isoforms. CD47 interact with integrins, Thrombospondin-1 (TSP-1), cell surface gylcoproteins SIRPα and SIRPβ. Owing to these interactions, CD47 plays an important role in lymphocyte homeostasis, maturation and activation of dendritic cells, cellular transmigration, and phagocytosis of cells. Expression of CD47 is found ubiquitously. CD47 is highly expressed on many different types of cancer and is considered as potential applications of CD47/SIRPα therapeutic blocking therapies.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

MER6, Integrin-associated protein (IAP), Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3

Detailed product information

Technical specifications

CloneREAL250
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD47
Alternative names of antigenMER6, Integrin-associated protein (IAP), Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3
Distribution of antigenubiquitous, prostate
RRIDAB_2877045

References for CD47 Antibody, anti-human, REAdye_lease™

Publications

  1. Demeure, C. E. et al. (2000) CD47 engagement inhibits cytokine production and maturation of human dendritic cells. J. Immunol. 164: 2193-2199
  2. Kusakari, S. et al. (2008) Trans-endocytosis of CD47 and SHPS-1 and its role in regulation of the CD47–SHPS-1 system. J. Cell. Sci. 121: 1213-1223
  3. Burger, P. et al. (2012) CD47 functions as a molecular switch for erythrocyte phagocytosis. Blood 119: 5512-5521
  4. Weiskopf, K. (2017) Cancer immunotherapy targeting the CD47/SIRPα axis. Eur. J. Cancer 76: 100-109

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