Clone:
REA312
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Membrane cofactor protein (MCP), AHUS2, MCP, MIC10, TLX, TRA2.10

Extended validation for CD46 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA312
E4.3++
8E2++
MEM-258-
TRA-2-10++
Cells were incubated with an excess of purified unconjugated CD46 (REA312) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD46 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD46-PE (REA312, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD46 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD46-PE (REA312, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD46 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD46-PE (REA312, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of human CD46 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD46-PE, clone (REA312). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of human CD46 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD46-PE, clone (REA312). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD46 Antibody, anti-human, REAfinity™

Overview

Clone REA312 recognizes the human CD46 antigen, a type I transmembrane glycoprotein, which is also known as the membrane cofactor protein (MCP). At least fourteen different transcript variants encoding different isoforms have been found for CD46. It is expressed by a broad range of tissues including leukocytes, platelets, epithelial cells, and fibroblasts, but not by erythrocytes. CD46 acts as a co-factor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It is involved in the fusion of the spermatozoa with the oocyte during fertilization. Additionally, CD46 functions as a receptor for measles virus, human herpesvirus, and human adenovirus. It is a potent costimulator for the induction of interferon-γ secreting effector T helper type 1 cells and their subsequent switch into interleukin 10 producing regulatory T cells.
Additional information: Clone REA312 displays negligible binding to Fc receptors.

Alternative names

Membrane cofactor protein (MCP), AHUS2, MCP, MIC10, TLX, TRA2.10

Detailed product information

Technical specifications

CloneREA312
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD46
Alternative names of antigenMembrane cofactor protein (MCP), AHUS2, MCP, MIC10, TLX, TRA2.10
Molecular mass of antigen [kDa]40
Distribution of antigenepithelial cells, fibroblasts, platelets, leukocytes
Entrez Gene ID4179
RRIDAB_2658391, AB_2658392, AB_2658393, AB_2658394, AB_2658395, AB_2905035, AB_2905034, AB_2905037, AB_2905036, AB_2922109, AB_2922106, AB_2922291, AB_2922285, AB_2658383, AB_2658384, AB_2658385, AB_2658388, AB_2658389, AB_2658390, AB_2658382

Resources for CD46 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD46 Antibody, anti-human, REAfinity™

Publications

  1. Le Friec, G. et al. (2012) The CD46-Jagged1 interaction is critical for human Tʜ1 immunity. Nat Immunol 13(12): 1213-1221
  2. Dörig, R. E. et al. (1993) The human CD46 molecule is a receptor for measles virus (Edmonston strain). Cell 75(2): 295-305
  3. Purcell, D. F. et al. (1991) Alternatively spliced RNAs encode several isoforms of CD46 (MCP), a regulator of complement activation. Immunogenetics 33(5-6): 335-344

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