Rat CD45RA MicroBeads were developed for positive selection or depletion of B cells from blood, peripheral lymphoid organs, or from non-lymphoid tissues.

Data and images for CD45RA MicroBeads, rat

Figures

Figure 1

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Isolation of B cells from rat spleen using CD45RA MicroBeads, a MiniMACS™ Separator, and an MS Column.

Figure 1

Isolation of B cells from rat spleen using CD45RA MicroBeads, a MiniMACS™ Separator, and an MS Column.

Specifications for CD45RA MicroBeads, rat

Overview

Rat CD45RA MicroBeads were developed for positive selection or depletion of B cells from blood, peripheral lymphoid organs, or from non-lymphoid tissues.

Detailed product information

Background information

The CD45RA isoform is B cell lineage–specific. It is neither expressed on thymocytes nor on any peripheral T cell subset. CD45RA was not detected on B cell progenitors in bone marrow.

Downstream applications

B cells separated with CD45RA MicroBeads can be used for analysis of B cell migration into non-lymphoid organs after infections, for evaluation of B cell responses after allograft transplantation, or to study the role of B cells in autoimmune diseases, such as arthritis or diabetes. Isolated B cells were used for adoptive transfer experiments to study autoimmunity and tolerance induction after xenogeneic thymus transplantation.
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Furthermore, isolated B cells were used for molecular analysis.
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Columns

For positive selection: MS, LS, XS, or autoMACS
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Columns. For depletion: LD, D, or autoMACS Columns.

References for CD45RA MicroBeads, rat

Publications

  1. Yan, Y. et al. (2003) Pathogenesis of autoimmunity after xenogeneic thymus transplantation. J. Immunol. 170: 5936-5946
  2. Lombardi, M. S. et al. (2001) Adjuvant arthritis induces down-regulation of G protein-coupled receptor kinases in the immune system. J. Immunol. 166: 1635-1640