Clone:
REAL164
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC, MICS, IHC, IF
Alternative names:
Ptprc, B220, CD45, CD45R, GP180, L-CA, LY5, T200

Extended validation for CD45RA Antibody, anti-human,
REAlease
®

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD45RA knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD45RA-PE (REAL164, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD45RA knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD45RA-PE (REAL164, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD45RA knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD45RA-PE (REAL164, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD45RA knockout cells. Wild type (red) and knockout cells (blue) were stained with CD45RA-PE, clone (REA164). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD45RA knockout cells. Wild type (red) and knockout cells (blue) were stained with CD45RA-PE, clone (REA164). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD45RA Antibody, anti-human,
REAlease
®

Overview

Clone REAL164 is an antibody fragment derived from the full CD45RA antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL164 recognizes the human CD45RA antigen which is expressed on naive CD4
+
and CD8
+
T cells as well as on CD8
+
effector T cells. CD45RA is also present on subsets of B and NK cells and on plasmacytoid dendritic cells. The CD45RA antibody recognizes the 220 kDa isoform of the leukocyte common antigen (LCA), a transmembrane tyrosine phosphatase.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

Ptprc, B220, CD45, CD45R, GP180, L-CA, LY5, T200

Detailed product information

Technical specifications

CloneREAL164
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Specieshuman
Cross-reactivity
cynomolgus monkey (
Macaca fascicularis
)
,
rhesus monkey (
Macaca mulatta
)
AntigenCD45RA
Alternative names of antigenPtprc, B220, CD45, CD45R, GP180, L-CA, LY5, T200
Distribution of antigenT cells, B cells, NK cells, dendritic cells, CD4+ T cells, CD8+ T cells, plasmacytoid dendritic cells
RRIDAB_2751966, AB_2751920, AB_2819366, AB_2819365, AB_2784220, AB_2751072

References for CD45RA Antibody, anti-human,
REAlease
®

Publications

  1. Cantisan, S. et al. (2010)
    CD45RA expression on HCMV-specific effector memory CD8
    +
    T cells is associated with the duration and intensity of HCMV replication after transplantation.
    Clin. Immunol. 137(1): 81-88
  2. Baba, N. et al. (2010)
    Interplay between CD45RA
    +
    regulatory T cells and TNF-α in the regulation of human Tʜ17 differentiation.
    Int. Immunol. 22: 237-244
  3. Braun, T. et al. (2012)
    Effector CD4
    +
    CD45RA
    CD25
    bright
    Foxp3
    bright
    regulatory T cell (eTreg) distribution is significantly impaired in chronic myelomonocytic leukemia (CMML) and correlates with TET 2 mutational status.
    Blood 120: 2808-2808

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