Clone:
REA450
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
Ptprc, T200, L-CA, LCA, RT7, CD45

Extended validation for CD45R Antibody, anti-rat, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA450
HIS24++
Cells were incubated with an excess of purified unconjugated CD45R (REA450) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD45R. Splenocytes from Wistar rat were stained with CD45R antibodies and with a suitable counterstaining. As a control, CD45R antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD45R. Splenocytes from Wistar rat were stained with CD45R antibodies and with a suitable counterstaining. As a control, CD45R antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD45R. Splenocytes from Wistar rat were stained with CD45R antibodies and with a suitable counterstaining. As a control, CD45R antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD45R. Splenocytes from Wistar rat were stained with CD45R antibodies and with a suitable counterstaining. As a control, CD45R antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD45R (REA450). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD45R (REA450). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD45R (REA450). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD45R Antibody, anti-rat, REAfinity™

Overview

Clone REA450 recognizes the rat CD45R antigen, an molecule which is expressed on B lymphocytes throughout their development from early pro–B stages onwards and is down-regulated upon terminal differentiation to plasma cells. Expression levels of CD45R are different among distinct B cell subpopulations and appears to be an indicator of maturational stages.
Additional information: Clone REA450 displays negligible binding to Fc receptors.

Alternative names

Ptprc, T200, L-CA, LCA, RT7, CD45

Detailed product information

Technical specifications

CloneREA450
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesrat
AntigenCD45R
Alternative names of antigenPtprc, T200, L-CA, LCA, RT7, CD45
Molecular mass of antigen [kDa]141
Distribution of antigenB cells, lymphocytes, plasma cells
Entrez Gene ID24699
RRIDAB_2784213, AB_2658294, AB_2658295, AB_2658296, AB_2658297, AB_2658298, AB_2658299, AB_2658300, AB_2658301, AB_2658302, AB_2658303, AB_2784214

References for CD45R Antibody, anti-rat, REAfinity™

Publications

  1. Opstelten, D. et al. (1986) B lymphocyte-associated antigens on terminal deoxynucleotidyl transferase-positive cells and pre-B cells in bone marrow of the rat. J. Immunol. 137(1): 76-84
  2. Kroese, F. G. et al. (1987) B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage-associated antigens. Eur. J. Immunol. 17(7): 921-928
  3. Kroese, F. G. et al. (1990) The rat B cell system: the anatomical localization of flow cytometry-defined B cell subpopulations. Eur. J. Immunol. 20(7): 1527-1534

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