Clone:
A20
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ
Applications:
FC
Alternative names:
Ptprc, B220, CD45, CD45R, L-CA, Ly-5, Lyt-4, T200, loc, Ly-5.1

Extended validation for CD45.1 Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with A20
REA1179++
REAL469++
Cells were incubated with an excess of purified unconjugated CD45.1 (A20) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD45.1 Antibody, anti-mouse

Overview

Clone A20 reacts with the murine CD45.1 alloantigen of CD45, a membrane glycoprotein also known as leukocyte common antigen or Ly-5. CD45.1 is expressed by all leukocytes in mouse strains SJL/J, STS/A, RIII, and DA. CD45 is a member of the protein tyrosine phosphatase family and is involved in the regulation of T and B cell antigen receptor signaling. Antibody clone A20 does not react with the CD45.2 alloantigen expressed by most mouse strains.

Alternative names

Ptprc, B220, CD45, CD45R, L-CA, Ly-5, Lyt-4, T200, loc, Ly-5.1

Detailed product information

Technical specifications

CloneA20
Clonalitymonoclonal
Isotypemouse IgG2aκ
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Speciesmouse
AntigenCD45.1
Alternative names of antigenPtprc, B220, CD45, CD45R, L-CA, Ly-5, Lyt-4, T200, loc, Ly-5.1
Molecular mass of antigen [kDa]142
Distribution of antigenB cells, granulocytes, Langerhans cells, macrophages, mast cells, monocytes, T cells, stem cells, leukocytes, lymphocytes, basophils, hematopoietic stem and progenitor cells, plasma cells, thymocytes
Entrez Gene ID19264
RRIDAB_2857776, AB_2660703, AB_2660704, AB_2660705, AB_2660706, AB_2660707, AB_2660708, AB_2660709, AB_2660710, AB_2660711, AB_2660712, AB_2660713, AB_2857674

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