Clone:
IM7.8.1
Type of antibody:
Primary antibodies
Isotype:
rat IgG2bκ
Applications:
FC, MICS, IHC, IF
Alternative names:
HERMES, Ly-24, Pgp-1, CD44s, EMCR III, H-CAM

Extended validation for CD44 Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with IM7.8.1
IM7++
REA664++
Cells were incubated with an excess of purified unconjugated CD44 (IM7.8.1) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD44 Antibody, anti-mouse

Overview

Mouse CD44 is also known as Pgp-1, HCELL, HUTCH-I, HCAM, HERMES, and Ly-24. Clone IM7 reacts with murine CD44, an 80–95 kDa membrane glycoprotein. It is expressed by a variety of hematopoetic and non-hematopoetic cells. Different isoforms are generated by alternative splicing. Clone IM7 reacts with all isoforms and both allelic forms of CD44. CD44 functions as a receptor for hyaluronic acid (HA) and mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). CD44 expression is upregulated on naive T cells upon activation and high CD44 expression is maintained on memory T cells. Therefore, CD44 is often used to discriminate CD44
high
antigen-experienced T cells from CD44
low
(CD62L
+
) naive T cells.

Alternative names

HERMES, Ly-24, Pgp-1, CD44s, EMCR III, H-CAM

Detailed product information

Technical specifications

CloneIM7.8.1
Clonalitymonoclonal
Isotyperat IgG2bκ
Isotype controlIsotype Control Antibody, rat IgG2b
Hostrat
Type of antibodyPrimary antibodies
Speciesmouse, non-human primate, other
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
,
chimpanzee (
Pan troglodytes
)
, baboon, human, pig, cow, horse, cat, dog
AntigenCD44
Alternative names of antigenHERMES, Ly-24, Pgp-1, CD44s, EMCR III, H-CAM
Molecular mass of antigen [kDa]83
Distribution of antigenendothelial cells, T cells, red blood cells, stem cells, leukocytes, lymphocytes, cancer stem cells, CNS cells, mesenchymal stem cells, plasma cells, ES and iPS cells, bone marrow, kidney, skeletal muscle, skin, lymphocytes, endothelial cells, T cells, red blood cells, stem cells, leukocytes, cancer stem cells, CNS cells, mesenchymal stem cells, plasma cells, ES and iPS cells, bone marrow, kidney, skeletal muscle, skin
Entrez Gene ID12505
RRIDAB_2727594, AB_2819675, AB_2658180, AB_2658181, AB_2658183, AB_2658186, AB_2658187, AB_2658190, AB_2658191, AB_2727570

References for CD44 Antibody, anti-mouse

Publications

  1. Ponta, H. et al. (2003) CD44: From adhesion molecules to signalling regulators. Nat. Rev. Mol. Cell Biol. 4(1): 33-45

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