Clone:
REA833
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Spn, Leukosialin, GALGP, gpL115, Lsn, Sialophorin

Extended validation for CD43 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD43 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD43-PE (REA833, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD43 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD43-PE (REA833, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD43 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD43-PE (REA833, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of human CD43 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD43-PE, clone (REA833). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of human CD43 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD43-PE, clone (REA833). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD43 Antibody, anti-human, REAfinity™

Overview

Clone REA833 recognizes the human CD43 antigen, also known as leukosialin, a cell surface sialoglycoprotein that is involved in cell adhesion, differentiation, and activation. The CD43 antigen is expressed on most leukocytes, i.e., T cells, NK cells, granulocytes, monocytes, and macrophages, on hematopoietic stem cells and on platelets, but not on erythrocytes. Among B cells, CD43 is expressed on activated B cells and plasma cells but not on resting B cells, e.g., naive B cells. In bone marrow, CD43 is found on pro–B cells but is down-regulated during transition to the pre–B cell stage. CD43 is expressed on different myeloid and lymphoid tumors, including some B cell malignancies.
Additional information: Clone REA833 displays negligible binding to Fc receptors.

Alternative names

Spn, Leukosialin, GALGP, gpL115, Lsn, Sialophorin

Detailed product information

Technical specifications

CloneREA833
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
olive baboon (
Papio anubis
)
AntigenCD43
Alternative names of antigenSpn, Leukosialin, GALGP, gpL115, Lsn, Sialophorin
Molecular mass of antigen [kDa]38
Distribution of antigenB cells, granulocytes, lymphocytes, macrophages, megakaryocytes, stem cells, monocytes, NK cells, platelets, red blood cells, T cells, basophils, mesenchymal stem cells, neutrophils, plasma cells, thymocytes, bone marrow
Entrez Gene ID6693
RRIDAB_2658095, AB_2658096, AB_2658097, AB_2658098, AB_2658099, AB_2658100, AB_2658101, AB_2658102, AB_2658103, AB_2658104, AB_2658105, AB_2658106, AB_2658107, AB_2658108, AB_2658109, AB_2658110, AB_2658111, AB_2658112, AB_2658094

References for CD43 Antibody, anti-human, REAfinity™

Publications

  1. Whitehead, A. S. et al. (1989) Molecular characterization of sialophorin (CD43), the lymphocyte surface sialoglycoprotein defective in Wiskott-Aldrich syndrome. Proc. Natl. Acad. Sci. U.S.A. 86(8): 2819-2823
  2. Shelley, C. S. et al. (1990) Structure of the human sialophorin (CD43) gene. Identification of features atypical of genes encoding integral membrane proteins. Biochem. J. 270(3): 569-576
  3. Kudo, S. et al. (1991) A short, novel promoter sequence confers the expression of human leukosialin, a major sialoglycoprotein on leukocytes. J. Biol. Chem. 266(13): 8483-8489

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