Clone:
REA336
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IHC, IF
Alternative names:
ITGA2B, BDPLT16, BDPLT2, GP2B

Extended validation for CD41b Antibody, anti-human, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
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Flow cytometric comparison of different clones for CD41b. Human peripheral blood platelets were stained with CD41b antibodies and with a suitable counterstaining. As a control, CD41b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD41b. Human peripheral blood platelets were stained with CD41b antibodies and with a suitable counterstaining. As a control, CD41b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD41b. Human peripheral blood platelets were stained with CD41b antibodies and with a suitable counterstaining. As a control, CD41b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD41b. Human peripheral blood platelets were stained with CD41b antibodies and with a suitable counterstaining. As a control, CD41b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD41b (REA336). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD41b (REA336). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD41b (REA336). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD41b Antibody, anti-human, REAfinity™

Overview

Clone REA336 recognizes the human CD41b antigen, the β chain of integrin α-IIb (CD41). CD41 associates with CD61 to form the heterodimeric GPIIb/GPIIIa complex that is present on human megakaryocytes and platelets. The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin, as well as vitronectin and mediates platelets aggregation.
Additional information: Clone REA336 displays negligible binding to Fc receptors.

Alternative names

ITGA2B, BDPLT16, BDPLT2, GP2B

Detailed product information

Technical specifications

CloneREA336
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD41b
Alternative names of antigenITGA2B, BDPLT16, BDPLT2, GP2B
Molecular mass of antigen [kDa]110
Distribution of antigenmegakaryocytes, platelets
Entrez Gene ID3674
RRIDAB_2876975, AB_2658058, AB_2658059, AB_2658060, AB_2658061, AB_2658062, AB_2658063, AB_2658064, AB_2658065, AB_2658066, AB_2658067, AB_2876974

References for CD41b Antibody, anti-human, REAfinity™

Publications

  1. Gekas, C. et al. (2013) CD41 expression marks myeloid-biased adult hematopoietic stem cells and increases with age. Blood 121(22): 4463-4472
  2. Poncz, M. et al. (1987) Structure of the platelet membrane glycoprotein IIb. Homology to the alpha subunits of the vitronectin and fibronectin membrane receptors. J. Biol. Chem. 262(18): 8476-8482
  3. Corbel, C. et al. (2005) (alpha)IIb integrin, a novel marker for hemopoietic progenitor cells. Int. J. Dev. Biol. 49(2-3): 279-284

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