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CD4 +CD25 + regulatory T cells were isolated from human PBMCs. The cells were fluorescently stained as indicated and analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to CD4 expression except for the CD25-APC vs. CD4 (VIT4)-VioBlue dot plot. |
PBMCs before separation |
CD4 +CD25 + Regulatory T Cell Isolation Kit | CD4 +CD25 + Regulatory T Cell Isolation Kit |
Pre-enriched CD4+ cells |
CD4 +CD25 + Regulatory T Cell Isolation Kit | |
Isolated CD4+CD25+ regulatory T cells |
CD4 +CD25 + Regulatory T Cell Isolation Kit | CD4 +CD25 + Regulatory T Cell Isolation Kit |
CD4 +CD25 + Regulatory T Cell Isolation Kit |
CD4 +CD25 + regulatory T cells were isolated from human PBMCs. The cells were fluorescently stained as indicated and analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to CD4 expression except for the CD25-APC vs. CD4 (VIT4)-VioBlue dot plot. |
PBMCs before separation |
CD4 +CD25 + Regulatory T Cell Isolation Kit | CD4 +CD25 + Regulatory T Cell Isolation Kit |
Pre-enriched CD4+ cells |
CD4 +CD25 + Regulatory T Cell Isolation Kit | |
Isolated CD4+CD25+ regulatory T cells |
CD4 +CD25 + Regulatory T Cell Isolation Kit | CD4 +CD25 + Regulatory T Cell Isolation Kit |
CD4 +CD25 + Regulatory T Cell Isolation Kit |
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