CD4
⁺
CD25
⁺
CD45RA
⁺
Regulatory T Cell Isolation Kit
, human

Name: CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kit, human
Availability: InStock

CD4
⁺
CD25
⁺
CD45RA
⁺
Regulatory T Cell Isolation Kit
, human

The CD4

⁺

CD25

⁺

CD45RA

⁺

Regulatory T Cell Isolation Kit was developed for the isolation of CD45RA

⁺

regulatory T cells (Treg) from human PBMCs in a two-step procedure.

Product data and images for CD4
⁺
CD25
⁺
CD45RA
⁺
Regulatory T Cell Isolation Kit
, human

Figures

Figure 1

CD4

⁺

CD25

⁺

CD45RA

⁺

regulatory T cells were isolated from human PBMCs by using the CD4

⁺

CD25

⁺

CD45RA

⁺

Regulatory T Cell Isolation Kit, an LD, and two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. The isolated cells were fluorescently stained with CD4, CD25, and either CD45RA or Anti-FoxP3 antibodies. Dot plots show data gated on viable lymphocytes (C) or viable CD4

⁺

lymphocytes (A, B).

Viable CD4⁺ lymphocytes

View details CD4 ⁺ CD25 ⁺ CD45RA ⁺ Regulatory T Cell Isolation Kit , human Figure 1 CD4 ⁺ CD25 ⁺ CD45RA ⁺ regulatory T cells were isolated from human PBMCs by using the CD4 ⁺ CD25 ⁺ CD45RA ⁺ Regulatory T Cell Isolation Kit, an LD, and two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. The isolated cells were fluorescently stained with CD4, CD25, and either CD45RA or Anti-FoxP3 antibodies. Dot plots show data gated on viable lymphocytes (C) or viable CD4 ⁺ lymphocytes (A, B).	View details CD4 ⁺ CD25 ⁺ CD45RA ⁺ Regulatory T Cell Isolation Kit , human Figure 1 CD4 ⁺ CD25 ⁺ CD45RA ⁺ regulatory T cells were isolated from human PBMCs by using the CD4 ⁺ CD25 ⁺ CD45RA ⁺ Regulatory T Cell Isolation Kit, an LD, and two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. The isolated cells were fluorescently stained with CD4, CD25, and either CD45RA or Anti-FoxP3 antibodies. Dot plots show data gated on viable lymphocytes (C) or viable CD4 ⁺ lymphocytes (A, B).
Viable lymphocytes
View details CD4 ⁺ CD25 ⁺ CD45RA ⁺ Regulatory T Cell Isolation Kit , human Figure 1 CD4 ⁺ CD25 ⁺ CD45RA ⁺ regulatory T cells were isolated from human PBMCs by using the CD4 ⁺ CD25 ⁺ CD45RA ⁺ Regulatory T Cell Isolation Kit, an LD, and two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. The isolated cells were fluorescently stained with CD4, CD25, and either CD45RA or Anti-FoxP3 antibodies. Dot plots show data gated on viable lymphocytes (C) or viable CD4 ⁺ lymphocytes (A, B).

Specifications for CD4
⁺
CD25
⁺
CD45RA
⁺
Regulatory T Cell Isolation Kit
, human

Overview

The CD4

⁺

CD25

⁺

CD45RA

⁺

Regulatory T Cell Isolation Kit was developed for the isolation of CD45RA

⁺

regulatory T cells (Treg) from human PBMCs in a two-step procedure.

Detailed product information

Background information

Regulatory CD4

⁺

CD25

⁺

T cells isolated from the CD45RA

⁺

naive T cell compartment were shown to be optimal for

in vitro

expansion. These expanded Treg cells maintained the FoxP3

⁺

phenotype and their suppressive activity, whereas CD45RA

^–

regulatory T cells from the memory T cell compartment lost FoxP3 expression and showed only modest suppressive activity.

¹

Detailed separation procedure

The isolation is performed in a two-step procedure. First, non-CD4

⁺

and memory T cells are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. The labeled cells are subsequently depleted over a MACS

^®

Column. In the second step, the flow-through fraction of pre-enriched CD4

⁺

CD45RA

⁺

T cells is labeled with CD25 MicroBeads for subsequent positive selection of CD4

⁺

CD25

⁺

CD45RA

⁺

T cells.

Downstream applications

CD4

⁺

CD25

⁺

CD45RA

⁺

regulatory T cells can be isolated from human PBMCs with this kit, for example, for further phenotypical or functional characterization and expansion.

Columns

For the first magnetic separation (depletion): LD or autoMACS

^®

Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Resources for CD4
⁺
CD25
⁺
CD45RA
⁺
Regulatory T Cell Isolation Kit
, human

Documents and Protocols

Brochures/posters

Regulatory T cell research

References for CD4
⁺
CD25
⁺
CD45RA
⁺
Regulatory T Cell Isolation Kit
, human

Publications

Hoffmann, P. et al. (2006)
Only the CD45RA
⁺
subpopulation of CD4
⁺
CD25
^high
T cells gives rise to homogeneous regulatory T-cell lines upon
in vitro
expansion.
Blood 108: 4260-4267

Data and images