Clone:
IB6
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ
Applications:
FC, MICS, IF, IHC, ICC
Alternative names:
ADPRC 1, ADPRC1, T10

Extended validation for CD38 Antibody, anti-human

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
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Fluorescence microscopy image of CD38 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD38-PE (IB6, red) and counterstained with DRAQ5 (blue) as DNA stain.
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Fluorescence microscopy image of CD38 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD38-PE (IB6, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD38 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD38-PE (IB6, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD38 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD38-PE, clone (IB6). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD38 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD38-PE, clone (IB6). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD38. Human peripheral blood mononuclear cells (PBMCs) were stained with CD38antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD38. Human peripheral blood mononuclear cells (PBMCs) were stained with CD38antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD38. Human peripheral blood mononuclear cells (PBMCs) were stained with CD38antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD38. Human peripheral blood mononuclear cells (PBMCs) were stained with CD38antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD38. Human peripheral blood mononuclear cells (PBMCs) were stained with CD38antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD38. Human peripheral blood mononuclear cells (PBMCs) were stained with CD38antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD38. Human peripheral blood mononuclear cells (PBMCs) were stained with CD38antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD38 (IB6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
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Comparison of staining pattern on non-fixed and fixed cells using CD38 (IB6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD38 (IB6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD38 Antibody, anti-human

Overview

Clone IB6 recognizes the human CD38 antigen, a single-chain type II trans­membrane glycoprotein with enzymatic activity. It is present on the majority of hematopoietic cells, prevalent during early differentiation and activation processes. Terminally differentiated B cells (plasma cells) express CD38 brightly. Furthermore, CD38 is constitutively expressed in several tissues, for example brain, muscle, and kidney.

Alternative names

ADPRC 1, ADPRC1, T10

Detailed product information

Technical specifications

CloneIB6
Clonalitymonoclonal
Isotypemouse IgG2aκ
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD38
Alternative names of antigenADPRC 1, ADPRC1, T10
Molecular mass of antigen [kDa]34
Distribution of antigenB cells, leukocytes, lymphocytes, monocytes, myeloid leukemia cells, NK cells, red blood cells, T cells, basophils, plasma cells, thymocytes, bone marrow, brain, kidney, ovary, pancreas, placenta, skeletal muscle
Entrez Gene ID952
RRIDAB_2733640, AB_2733812, AB_2733813, AB_2733374, AB_2733375, AB_2733229, AB_2733230, AB_2733520, AB_2733521, AB_2733639

Reviews for CD38 Antibody, anti-human

Good CD38 Antibody from Miltenyi Biotec

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CD38-FITC, human (130-113-426)

Publishable results!

References for CD38 Antibody, anti-human

Publications

  1. Yoon-Sang, K. et al. (2010) Transduction of human primitive repopulating hematopoietic cells with lentiviral vectors pseudotyped with various envelope proteins. Mol. Ther. 18(7): 1310-1317

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