Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Alternative names:
HAVcr-2, TIMD-3

Extended validation for CD366 (TIM-3) Antibody, anti-human,


Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REAL226
Cells were incubated with an excess of purified unconjugated CD366 (TIM-3) (REAL226) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD366 (TIM-3) Antibody, anti-human,


Clone REAL226 is an antibody fragment derived from the full CD366 (TIM-3) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL226 recognizes the human CD366 antigen, a single-pass type I membrane protein which is also known as T cell immunoglobulin and mucin domain containing protein-3 (TIM-3) or Hepatitis A virus cellular receptor 2 (HAVcr-2). CD366 is expressed on activated T cells, especially Tʜ1 cells, monocytes, macrophages, and dendritic cells. It is an activation-induced inhibitory molecule involved in tolerance and shown to induce T cell exhaustion in chronic viral infection and cancers. CD366 has been proposed to inhibit Tʜ1-mediated immune responses and regulates Tʜ1 and Tʜ17 cytokine secretion. The interaction of CD366 and its ligand galectin-9 induces apoptosis of Tʜ1 cells.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

HAVcr-2, TIMD-3

Detailed product information

Technical specifications

Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
AntigenCD366 (TIM-3)
Alternative names of antigenHAVcr-2, TIMD-3
Distribution of antigendendritic cells, macrophages, monocytes, T cells
RRIDAB_2751534, AB_2784167, AB_2857791, AB_2857788, AB_2751545

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