Clone:
REA339
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
SLAMF6, KALI, KALIb, LY108, NTB-A, NTBA, SF2000, SLAM6, TCOM

Extended validation for CD352 (NTB-A) Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD352 (NTB-A) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD352 (NTB-A)-PE (REA339, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD352 (NTB-A) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD352 (NTB-A)-PE (REA339, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD352 (NTB-A) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD352 (NTB-A)-PE (REA339, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD352 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD352-PE, clone (REA339). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD352 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD352-PE, clone (REA339). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD352 (NTB-A) Antibody, anti-human, REAfinity™

Overview

Clone REA339 recognizes the human CD352 antigen, a 60 kDa single-pass type I membrane protein also known as SLAM family member 6 (SLAM6) or natural killer (NK), T, and B cell–antigen (NTB-A). CD352 belongs to the CD2 subfamily of the immunoglobulin superfamily and is expressed on NK, T, and B cells. It undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases. Monoclonal antibody-mediated cross-linking of CD352 results in the induction of NK-mediated cytotoxicity. CD352 also triggers cytolytic activity only in NK cells expressing high surface densities of natural cytotoxicity receptors. This suggests that CD352 may function as a coreceptor in the process of NK cell activation. Importantly, analysis of NK cells derived from patients with X-linked lymphoproliferative disease showed that the lack of SH2D1A protein profoundly affects the function of CD352.
Additional information: Clone REA339 displays negligible binding to Fc receptors.

Alternative names

SLAMF6, KALI, KALIb, LY108, NTB-A, NTBA, SF2000, SLAM6, TCOM

Detailed product information

Technical specifications

CloneREA339
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD352 (NTB-A)
Alternative names of antigenSLAMF6, KALI, KALIb, LY108, NTB-A, NTBA, SF2000, SLAM6, TCOM
Molecular mass of antigen [kDa]35
Distribution of antigenB cells, NK cells, T cells
Entrez Gene ID114836
RRIDAB_2752214, AB_2876972, AB_2876973, AB_2657670, AB_2657671, AB_2657672, AB_2657673, AB_2657674, AB_2657675, AB_2657676, AB_2657677, AB_2752217

References for CD352 (NTB-A) Antibody, anti-human, REAfinity™

Publications

  1. Katz, G. et al. (2014) SAP facilitates recruitment and activation of LCK at NTB-A receptors during restimulation-induced cell death. J. Immunol. 192(9): 4202-4209
  2. Bottino, C. et al. (2001) NTB-A [correction of GNTB-A], a novel SH2D1A-associated surface molecule contributing to the inability of natural killer cells to kill Epstein-Barr virus-infected B cells in X-linked lymphoproliferative disease. J. Exp. Med. 194(3): 235-246
  3. Valdez, P. A. et al. (2004) NTB-A, a new activating receptor in T cells that regulates autoimmune disease. J. Biol. Chem. 279(18): 18662-18669

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