Clone:
9E2
Type of antibody:
Primary antibodies, Functional-grade antibodies
Isotype:
mouse IgG1κ, mouse IgG1
Applications:
FC, FA
Alternative names:
NCR1, LY94, NKp46

Extended validation for CD335 (NKp46) Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 9E2
REA808++
195314++
Cells were incubated with an excess of purified unconjugated CD335 (NKp46) (9E2) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD335 (NKp46) (9E2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD335 (NKp46) (9E2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD335 (NKp46) (9E2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD335 (NKp46) Antibody, anti-human

Overview

Clone 9E2 recognizes the CD335 antigen, also known as NKp46. CD335 is a member of the natural cytotoxicity receptor (NCR) family which trigger cytotoxicity in NK cells. CD335 is directly involved in target cell recognition and lysis and is exclusively expressed on CD3
CD56
+
NK cells, suggesting it to be a universal marker for NK cells.

Alternative names

NCR1, LY94, NKp46

Detailed product information

Technical specifications

Clone9E2
Clonalitymonoclonal
Isotypemouse IgG1κ, mouse IgG1
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies, Functional-grade antibodies
Specieshuman
AntigenCD335 (NKp46)
Alternative names of antigenNCR1, LY94, NKp46
Molecular mass of antigen [kDa]32
Distribution of antigenNK cells
Entrez Gene ID9437
RRIDAB_2876904, AB_2876905, AB_2857835, AB_2857832, AB_10828922, AB_10828456, AB_2660317, AB_615081, AB_2660318, AB_2660319, AB_2660320, AB_2660321, AB_2660322, AB_1103213, AB_615080

Resources for CD335 (NKp46) Antibody, anti-human

Documents and Protocols

Reviews for CD335 (NKp46) Antibody, anti-human

Anti-Human NKp46 PE

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CD335 (NKp46)-PE, human (130-092-607)

Works well with other NK markers, would recommend.

Anti-Human NKp46 PE

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CD335 (NKp46)-PE, human (130-099-203)

Works well with other NK markers, would recommend.

References for CD335 (NKp46) Antibody, anti-human

Publications

  1. Pessino, A. et al. (1998) Molecular cloning of NKp46: A novel member of the immunoglobulin superfamily involved in triggering of natural cytotoxicity. J. Exp. Med. 188: 953-960
  2. Moretta, L. and Moretta, A. (2004) Unravelling natural killer function: Triggering and inhibitory human NK receptors. EMBO J. 23: 255-259
  3. Sivori, S. et al. (2000) 2B4 functions as a co-receptor in human NK cell activation. Eur. J. Immunol. 30: 787-793
  4. Moretta, A. et al. (2001) Activating receptors and coreceptors involved in human natural killer cell-mediated cytolysis. Annu. Rev. Immunol. 19: 197-223
  5. Lai, C. B. et al. (2012) Role of runt-related transcription factor 3 (RUNX3) in transcription regulation of natural cytotoxicity receptor 1 (NCR1/NKp46), an activating natural killer (NK) cell receptor. J. Biol. Chem. 287(10): 7324-7334

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