Clone:
REAL539
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
EGP40, Ep-CAM, KSA, MK-1, TROP1

Specifications for CD326 (EpCAM) Antibody, anti-human, REAdye_lease™

Overview

Clone REAL539 is an antibody fragment derived from the full CD326 (EpCAM) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL539 recognizes CD326, also known as human epithelial antigen (HEA), epithelial cell adhesion molecule (EpCAM), or epithelial-specific antigen (ESA) and is involved in cell adhesion. The CD326 antigen is broadly expressed on the basolateral surface of carcinoma and epithelial cells in tissues or on circulating tumor cells, and cancer stem cells, but is not found on melanoma, neuroblastoma, sarcoma, lymphoma, leukemia cells, or normal fibroblasts. Furthermore, it is suggested, that CD326 could be used as a surface marker for human ESCs.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

EGP40, Ep-CAM, KSA, MK-1, TROP1

Detailed product information

Technical specifications

CloneREAL539
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD326 (EpCAM)
Alternative names of antigenEGP40, Ep-CAM, KSA, MK-1, TROP1
Distribution of antigenepithelial cells, cancer stem cells, ES and iPS cells, ovarian cancer
RRIDAB_2857563, AB_2857560

References for CD326 (EpCAM) Antibody, anti-human, REAdye_lease™

Publications

  1. Moldenhauer, G. et al. (1987) Epithelium specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br. J. Cancer 56: 714-721
  2. Sheridan, C. et al. (2006)
    CD44
    +
    /CD24
    -
    breast cancer cells exhibit enhanced invasive properties: an early step necessary for metastasis.
    Breast Cancer Res. 8(5): R59
  3. Metsuyanim, S. et al. (2009) Expression of stem cell markers in the human fetal kidney. PLoS One 4(68): e6709
  4. Matsumoto, T. et al. (2017) Proliferating EpCAM-Positive Ductal Cells in the Inflamed Liver Give Rise to Hepatocellular Carcinoma. Cancer Res. 77(22): 6132-6143