Clone:
REAL260
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC, MICS, IHC, IF
Alternative names:
PECAM1, Pecam, EndoCAM, GPIIa

Extended validation for CD31 Antibody, anti-mouse,
REAlease
®

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REAL260
390++
MEC13.3-
693102-
REA784++
Cells were incubated with an excess of purified unconjugated CD31 (REAL260) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD31 Antibody, anti-mouse,
REAlease
®

Overview

Clone REAL260 is an antibody fragment derived from the full CD31 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL260 recognizes the cell surface protein CD31. The murine CD31 antigen is also known as PECAM-1. The encoded protein is a single-pass type I membrane protein containing six Ig-like C2-type (immunoglobulin-like) domains and functions as cell adhesion molecule. CD31 is present on mature endothelial cells as well as on most leukocyte subtypes and platelets where the expression level can vary. Besides its function in exhibiting adhesive properties, the protein is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

PECAM1, Pecam, EndoCAM, GPIIa

Detailed product information

Technical specifications

CloneREAL260
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD31
Alternative names of antigenPECAM1, Pecam, EndoCAM, GPIIa
Distribution of antigenendothelial cells, leukocytes, platelets
RRIDAB_2751301, AB_2784130, AB_2811322

References for CD31 Antibody, anti-mouse,
REAlease
®

Publications

  1. Xie, Y. and Muller, W. A. (1993) Molecular cloning and adhesive properties of murine platelet/endothelial cell adhesion molecule 1. Proc. Natl. Acad. Sci. U.S.A. 90(12): 5569-5573
  2. Woodfin, A. et al. (2007) PECAM-1: a multi-functional molecule in inflammation and vascular biology. Arterioscler. Thromb. Vasc. Biol. 27(12): 2514-2523
  3. van Buul, J. D. and Hordijk, P. L. (2008) Endothelial signaling by Ig-like cell adhesion molecules. Transfus. Clin. Biol. 15(1-2): 3-6

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REAlease
®

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