Clone:
REA730
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MC
Alternative names:
PECAM-1, EndoCAM, GPIIa, PECA1

Extended validation for CD31 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA730
MBC.78:2-
L133.1-
WM59++
MEM-05-
1F11-
M89D3-
Cells were incubated with an excess of purified unconjugated CD31 (REA730) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD31 Antibody, anti-human, REAfinity™

Overview

Clone REA730 recognizes the human CD31 antigen, also known as PECAM-1. CD31 is a member of the immunoglobulin superfamily and is involved in leukocyte transmigration, angiogenesis, and integrin activation. CD31 is a 120–140 kDa single-chain transmembrane glycoprotein expressed on endothelial cells, monocytes, platelets, granulocytes, and several lymphocyte subsets. During maturation of CD4
+
T cells, the expression of CD31 changes: CD4
+
recent thymic emigrants (RTEs) express CD31 in contrast to central naive T cells. CD31 is highly expressed on endothelial cells, especially at their juncture.
Additional information: Clone REA730 displays negligible binding to Fc receptors.

Alternative names

PECAM-1, EndoCAM, GPIIa, PECA1

Detailed product information

Technical specifications

CloneREA730
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD31
Alternative names of antigenPECAM-1, EndoCAM, GPIIa, PECA1
Molecular mass of antigen [kDa]80
Distribution of antigenplatelets, B cells, NK cells, T cells, monocytes, granulocytes, endothelial cells
Entrez Gene ID5175
RRIDAB_2657279, AB_2657280, AB_2657281, AB_2657282, AB_2657283, AB_2657284, AB_2657285, AB_2657286, AB_2657287, AB_2657288, AB_2657289, AB_2657290, AB_2657291, AB_2657292, AB_2657293, AB_2657294, AB_2657295, AB_2819612, AB_2657278

References for CD31 Antibody, anti-human, REAfinity™

Publications

  1. Newman, P. J. et al. (1990) PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247(4947): 1219-1222
  2. Stockinger, H. et al. (1990) Molecular characterization and functional analysis of the leukocyte surface protein CD31. J. Immunol. 145(11): 3889-3897
  3. Kimmig, S. et al. (2002) Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood. J. Exp. Med. 195: 789-794

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