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Mouse ES cells (HM1) were differentiated into embryoid bodies (EBs). At day 5 EBs were dissociated with the Embryoid Body Dissociation Kit (# 130-096-348) and CD309 expressing cells were isolated using the CD309 (Flk-1) MicroBead Kit, an LS Column, and a MidiMACS™ Separator. After fluorescent labeling with Labeling Check Reagent‑APC (# 130-095-237) cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Unseparated cells | Isolated CD309 + cells |
CD309 (Flk-1) MicroBead Kit, mouseFigure 1Mouse ES cells (HM1) were differentiated into embryoid bodies (EBs). At day 5 EBs were dissociated with the Embryoid Body Dissociation Kit (# 130-096-348) and CD309 expressing cells were isolated using the CD309 (Flk-1) MicroBead Kit, an LS Column, and a MidiMACS™ Separator. After fluorescent labeling with Labeling Check Reagent‑APC (# 130-095-237) cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. | CD309 (Flk-1) MicroBead Kit, mouseFigure 1Mouse ES cells (HM1) were differentiated into embryoid bodies (EBs). At day 5 EBs were dissociated with the Embryoid Body Dissociation Kit (# 130-096-348) and CD309 expressing cells were isolated using the CD309 (Flk-1) MicroBead Kit, an LS Column, and a MidiMACS™ Separator. After fluorescent labeling with Labeling Check Reagent‑APC (# 130-095-237) cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Improved endothelial differentiation of isolated CD309 (Flk-1)+ cells: Unseparated or CD309-enriched cardiovascular progenitor cells (derived as in fig. 1) were subjected to endothelial differentiation in the presence of 50 ng/mL VEGF. Differentiation efficiency was evaluated by immunofluorescence using CD31-FITC (green) and DAPI counterstain (blue). |
Unseparated cells | Isolated CD309 + cells |
CD309 (Flk-1) MicroBead Kit, mouseFigure 2Improved endothelial differentiation of isolated CD309 (Flk-1)+ cells: Unseparated or CD309-enriched cardiovascular progenitor cells (derived as in fig. 1) were subjected to endothelial differentiation in the presence of 50 ng/mL VEGF. Differentiation efficiency was evaluated by immunofluorescence using CD31-FITC (green) and DAPI counterstain (blue). | CD309 (Flk-1) MicroBead Kit, mouseFigure 2Improved endothelial differentiation of isolated CD309 (Flk-1)+ cells: Unseparated or CD309-enriched cardiovascular progenitor cells (derived as in fig. 1) were subjected to endothelial differentiation in the presence of 50 ng/mL VEGF. Differentiation efficiency was evaluated by immunofluorescence using CD31-FITC (green) and DAPI counterstain (blue). |
Mouse ES cells (HM1) were differentiated into embryoid bodies (EBs). At day 5 EBs were dissociated with the Embryoid Body Dissociation Kit (# 130-096-348) and CD309 expressing cells were isolated using the CD309 (Flk-1) MicroBead Kit, an LS Column, and a MidiMACS™ Separator. After fluorescent labeling with Labeling Check Reagent‑APC (# 130-095-237) cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Unseparated cells | Isolated CD309 + cells |
CD309 (Flk-1) MicroBead Kit, mouseFigure 1Mouse ES cells (HM1) were differentiated into embryoid bodies (EBs). At day 5 EBs were dissociated with the Embryoid Body Dissociation Kit (# 130-096-348) and CD309 expressing cells were isolated using the CD309 (Flk-1) MicroBead Kit, an LS Column, and a MidiMACS™ Separator. After fluorescent labeling with Labeling Check Reagent‑APC (# 130-095-237) cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. | CD309 (Flk-1) MicroBead Kit, mouseFigure 1Mouse ES cells (HM1) were differentiated into embryoid bodies (EBs). At day 5 EBs were dissociated with the Embryoid Body Dissociation Kit (# 130-096-348) and CD309 expressing cells were isolated using the CD309 (Flk-1) MicroBead Kit, an LS Column, and a MidiMACS™ Separator. After fluorescent labeling with Labeling Check Reagent‑APC (# 130-095-237) cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Improved endothelial differentiation of isolated CD309 (Flk-1)+ cells: Unseparated or CD309-enriched cardiovascular progenitor cells (derived as in fig. 1) were subjected to endothelial differentiation in the presence of 50 ng/mL VEGF. Differentiation efficiency was evaluated by immunofluorescence using CD31-FITC (green) and DAPI counterstain (blue). |
Unseparated cells | Isolated CD309 + cells |
CD309 (Flk-1) MicroBead Kit, mouseFigure 2Improved endothelial differentiation of isolated CD309 (Flk-1)+ cells: Unseparated or CD309-enriched cardiovascular progenitor cells (derived as in fig. 1) were subjected to endothelial differentiation in the presence of 50 ng/mL VEGF. Differentiation efficiency was evaluated by immunofluorescence using CD31-FITC (green) and DAPI counterstain (blue). | CD309 (Flk-1) MicroBead Kit, mouseFigure 2Improved endothelial differentiation of isolated CD309 (Flk-1)+ cells: Unseparated or CD309-enriched cardiovascular progenitor cells (derived as in fig. 1) were subjected to endothelial differentiation in the presence of 50 ng/mL VEGF. Differentiation efficiency was evaluated by immunofluorescence using CD31-FITC (green) and DAPI counterstain (blue). |
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