Clone:
REAL1097
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
CD3-epsilon, CD3e, Leu4, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, IMD18, TCRE

Specifications for CD3 Antibody, anti-human, REAdye_lease™

Overview

Clone REAL1097 is an antibody fragment derived from the full CD3 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL1097 recognizes the human CD3 antigen, a single-pass type I membrane protein, also known as Leu4. CD3 is present on mature human T cells and related neoplasms, thymocytes, a subset of NK cells, and on Purkinje cells in cerebellum. The CD3 antigen is a complex of five invariable chains: γ, δ, ε, ζ, and η. It is associated with the T cell receptor (TCR) and is responsible for its signal transduction. Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes expressed on the surface of APCs. The T cell receives a signal transduced through the CD3 complex. Cytokines or other costimulatory signals are required in addition. Activated T cells can be used for any downstream processes, such as cytokine analysis or immunoprecipitation. Cells can also be transfected with high efficiency.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

CD3-epsilon, CD3e, Leu4, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, IMD18, TCRE

Detailed product information

Technical specifications

CloneREAL1097
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD3
Alternative names of antigenCD3-epsilon, CD3e, Leu4, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, IMD18, TCRE
Distribution of antigenleukocytes, NK cells, T cells, thymocytes

References for CD3 Antibody, anti-human, REAdye_lease™

Publications

  1. Gold, D. P. et al. (1986) Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex. Nature 321(6068): 431-434
  2. D'Acquisto, F. et al. (2011)
    CD3
    +
    CD4
    -
    CD8
    -
    (double negative) T cells: saviours or villains of the immune response?
    Biochem. Pharmacol. 82(4): 333-340
  3. Birnbaum, M. E. et al. (2014) Molecular architecture of the αβ T cell receptor-CD3 complex. Proc. Natl. Acad. Sci. U.S.A. 111(49): 17576-17581
  4. Żabińska, M. et al. (2016) CD3(+)CD8(+)CD28(-) T Lymphocytes in Patients with Lupus Nephritis. J Immunol Res. 2016: 1058165
  5. Lee, E.J. et al. (2017) CD3 and CD20 Immunohistochemical Staining Patterns of Bone Marrow-Infiltrating Malignant Lymphoma Cells. Ann Clin Lab Sci . 47(2): 136-143