Clone:
REA1216
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
TNFRSF12A, FN14, TWEAKR

Extended validation for CD266 (FN14) Antibody, anti-human/mouse, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD266 (FN14) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD266 (FN14)-PE (REA1216, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD266 (FN14) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD266 (FN14)-PE (REA1216, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD266 (FN14) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD266 (FN14)-PE (REA1216, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD266 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD266-PE, clone (REA1216). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD266 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD266-PE, clone (REA1216). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD266 (FN14) Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA1216 recognizes CD266, also known as FN14, tumor necrosis factor receptor superfamily member 12A (TNFRSF12A), or tweak-receptor (TWEAKR). CD266 is a 14 kDa type I transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily and binds to TWEAK, a proinflammatory cytokine, as its only known ligand. The cytoplasmic tail of CD266 contains a TRAF binding motif and binds to TRAF1, TRAF2, TRAF3, and TRAF5. Both TWEAK dependent and independent CD266 signaling lead to activation of the NF-κB pathway. TWEAK independent signaling is shown when CD266 is ectopically overexpressed
in vitro
. Expression of CD266 is found on a wide variety of cells except for primary T and B cells. Elevated expression is often found in response to several growth factors, cytokines, hormones, after tissue injury, and in solid tumors such as glioblastoma multiforme tumors. Varied functions of TWEAK signaling include promotion of wound healing and regeneration, to influence the transition from innate to adaptive immunity and regulation of cell death and tumor progression.
Additional information: Clone REA1216 displays negligible binding to Fc receptors.

Alternative names

TNFRSF12A, FN14, TWEAKR

Detailed product information

Technical specifications

CloneREA1216
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse
AntigenCD266 (FN14)
Alternative names of antigenTNFRSF12A, FN14, TWEAKR
Molecular mass of antigen [kDa]11
Distribution of antigenlung, heart, kidney, muscle, pancreas
Entrez Gene ID51330
RRIDAB_2811503, AB_2811510, AB_2811504, AB_2811511, AB_2811505, AB_2811512, AB_2811506, AB_2811513, AB_2811507, AB_2811514, AB_2811508, AB_2811509

Resources for CD266 (FN14) Antibody, anti-human/mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD266 (FN14) Antibody, anti-human/mouse, REAfinity™

Publications

  1. Nakayama, M. et al. (2003) Fibroblast growth factor-inducible 14 mediates multiple pathways of TWEAK-induced cell death. J Immunol 170: 341-348
  2. Vince, J. E. et al. (2006) TWEAK shall inherit the earth. Cell Death Differ. 13(11): 1842-1844
  3. Winkles, J. A. (2008) The TWEAK-Fn14 cytokine-receptor axis: discovery, biology and therapeutic targeting. Nat. Rev. Drug Discov. 7(5): 411-425

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