Clone:
REAL537
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC
Alternative names:
IL-2-RA, IL-2R subunit alpha, p55

Specifications for CD25 Antibody, anti-mouse,
REAlease
®

Overview

Clone REAL537 is an antibody fragment derived from the full CD25 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL537 recognizes the mouse CD25 antigen, the α chain of the IL-2 receptor (IL-2R). IL-2Rα associates with the β chain (CD122) and the γ chain (CD132) to form the functional IL-2R complex. IL-2Rα is expressed on CD4
+
CD25
+
regulatory T cells, activated T and B cells, and to a lesser extent on activated dendritic cells. It is also transiently expressed during T and B cell development. Binding of the CD25 antibody does not impede the binding of IL-2 to its receptor. In combination with CD4, CD25 antibody can be used to identify regulatory T cells (Tregs).
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

IL-2-RA, IL-2R subunit alpha, p55

Detailed product information

Technical specifications

CloneREAL537
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD25
Alternative names of antigenIL-2-RA, IL-2R subunit alpha, p55
Distribution of antigenT cells, B cells, dendritic cells