Clone:
REAL1225
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
2B4, NAIL, NKR2B4, Nmrk, SLAMF4

Specifications for CD244 (2B4) Antibody, anti-human, REAdye_lease™

Overview

Clone REAL1225 is an antibody fragment derived from the full CD244 (2B4) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL1225 recognizes CD244 (2B4), a type I transmembrane receptor belonging to the CD2 subfamily of the Ig superfamily. Expression of CD244 (2B4) is found on all NK cells, large subset of CD8
+
T cells, a small fraction of CD4
+
T cells, and eosinophils. Human CD244 (2B4) upon ligand engagement activates NK cell cytotoxicity and production of IFN-γ. Activating function of human CD244 (2B4) requires recruitment of SH2 domain containing molecule called SLAM associated protein (SAP). In humans, CD244 (2B4) has also been implicated as an inhibitory receptor such as in developing NK cells. In contrast, murine CD244 (2B4) is primarily involved in inhibiting NK cell functions. Two isoforms of human CD244 (2B4) exist on NK cells, h2B4-A and h2B4-B, which differ in their binding affinity to ligand CD48.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

2B4, NAIL, NKR2B4, Nmrk, SLAMF4

Detailed product information

Technical specifications

CloneREAL1225
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD244 (2B4)
Alternative names of antigen2B4, NAIL, NKR2B4, Nmrk, SLAMF4
Distribution of antigenNK cells, T cells, CD4+ T cells, CD8+ T cells, eosinophils

Resources for CD244 (2B4) Antibody, anti-human, REAdye_lease™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD244 (2B4) Antibody, anti-human, REAdye_lease™

Publications

  1. Sivori, S. et al. (2002) Early expression of triggering receptors and regulatory role of 2B4 in human natural killer cell precursors undergoing in vitro differentiation. Proc. Natl. Acad. Sci. U.S.A. 99: 4526-4531
  2. Vaidya, S. V. et al. (2006) Of mice and men: different functions of the murine and human 2B4 (CD244) receptor on NK cells. Immunol. Lett. 105(2): 180-184
  3. Munitz, A. et al. (2005) 2B4 (CD244) is expressed and functional on human eosinophils. J Immunol 174: 110-118
  4. Mathew, S. O. et al. (2009) Functional role of human NK cell receptor 2B4 (CD244) isoforms. Eur. J. Immunol. 39(6): 1632-1641

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