Clone:
REA175
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
GYPA, GPA, GPErik, GPSAT, HGpMiV, HGpMiXI, HGpSta(C), MN, MNS, PAS-2

Extended validation for CD235a (Glycophorin A) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA175
GA-RA (HIR2)+
HI264-
YTH89.1-
10F7MN-
CLB-ery-1 (AME-1)-
Cells were incubated with an excess of purified unconjugated CD235a (Glycophorin A) (REA175) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD235a (Glycophorin A) Antibody, anti-human, REAfinity™

Overview

Clone REA175 recognizes CD235a, commonly known as glycophorin A. CD235a is a single-pass transmembrane glycoprotein and is expressed on mature erythrocytes and erythroid precursor cells. It is one of the major sialoglycoprotein expressed on human red blood cells.
The CD235a (Glycophorin A) antibody has been tested by flow cytometric analysis of human peripheral blood cells according to the protocol. Binding of the antibody to red cells at high and moderate antibody concentration can cause cell agglutination, so it is recommended to titrate the antibody carefully for optimal performance in the assay of interest.
Additional information: Clone REA175 displays negligible binding to Fc receptors.

Alternative names

GYPA, GPA, GPErik, GPSAT, HGpMiV, HGpMiXI, HGpSta(C), MN, MNS, PAS-2

Detailed product information

Technical specifications

CloneREA175
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD235a (Glycophorin A)
Alternative names of antigenGYPA, GPA, GPErik, GPSAT, HGpMiV, HGpMiXI, HGpSta(C), MN, MNS, PAS-2
Molecular mass of antigen [kDa]14
Distribution of antigenred blood cells
Entrez Gene ID2993
RRIDAB_2728019, AB_2751527, AB_2751491, AB_2801777, AB_2801768, AB_2784083, AB_2784082, AB_2784085, AB_2784084, AB_2752159, AB_2752109, AB_2819396, AB_2819393, AB_2784081, AB_2784080, AB_2819397, AB_2819394, AB_2656511, AB_2728042

Resources for CD235a (Glycophorin A) Antibody, anti-human, REAfinity™

References for CD235a (Glycophorin A) Antibody, anti-human, REAfinity™

Publications

  1. Chasis, J. A. and Mohandas, N. (1992) Red blood cell glycophorins. Blood 80(8): 1869-1879

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