Clone:
M-L23.4
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ, mouse IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
FCER2, BLAST-2, CD23A, CLEC4J, FCE2, IGEBF, FcεRII

Extended validation for CD23 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with M-L23.4
REA1222++
EVCV-5+
EBCV-2+
M-L233+
9P25+
Cells were incubated with an excess of purified unconjugated CD23 (M-L23.4) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD23 (M-L23.4). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD23 (M-L23.4). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD23 (M-L23.4). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD23 Antibody, anti-human

Overview

CD23 is a 45 kDa type II transmembrane glycoprotein that belongs to the immunoglobulin family. It is expressed on most mature B cells, monocytes, follicular dendritic cells, and at low levels on T cells, Langerhans cells, eosinophils, and platelets. CD23 is thought to play a role in the regulation of B cell activation and IgE response. It is up-regulated upon B cell activation. The proteolytic cleavage of membrane CD23 can result in the release of soluble forms of the antigen (sCD23) with a reported biological activity.

Alternative names

FCER2, BLAST-2, CD23A, CLEC4J, FCE2, IGEBF, FcεRII

Detailed product information

Technical specifications

CloneM-L23.4
Clonalitymonoclonal
Isotypemouse IgG1κ, mouse IgG1
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD23
Alternative names of antigenFCER2, BLAST-2, CD23A, CLEC4J, FCE2, IGEBF, FcεRII
Molecular mass of antigen [kDa]36
Distribution of antigenB cells, dendritic cells, endothelial cells, Langerhans cells, macrophages, monocytes, NK cells, platelets, T cells, eosinophils, neutrophils, thymocytes
Entrez Gene ID2208
RRIDAB_2751599, AB_2857651, AB_2857623, AB_2819599, AB_2819584, AB_2659801, AB_2659799, AB_2659800, AB_10828801, AB_2751645

Resources for CD23 Antibody, anti-human

Certificates

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