Clone:
REA776
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
LAG-3, Ly66

Extended validation for CD223 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA776
631501-
C9B7W++
Cells were incubated with an excess of purified unconjugated CD223 (REA776) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD223 (REA776). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD223 (REA776). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD223 (REA776). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD223 Antibody, anti-mouse, REAfinity™

Overview

Clone REA776 recognizes an epitope within the D2 domain of murine CD233, also known as LAG-3, which is a 70 kDa high affinity MHC class II ligand. It is expressed on activated CD4
+
and CD8
+
α/β T cells, on a subset of NK cells, B cells and plasmacytoid dendritic cells. It is proposed, that LAG-3 could be used as a marker of T cell induced B cell activation.
In association with the CD3 complex CD223 is a negative regulatory molecule to inhibit TCR-mediated signaling and to regulate homeostatic T cell expansion.
Additional information: Clone REA776 displays negligible binding to Fc receptors

Alternative names

LAG-3, Ly66

Detailed product information

Technical specifications

CloneREA776
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD223
Alternative names of antigenLAG-3, Ly66
Molecular mass of antigen [kDa]55
Distribution of antigenB cells, dendritic cells, T cells, NK cells
Entrez Gene ID16768
RRIDAB_2656413, AB_2656414, AB_2656415, AB_2656416, AB_2656417, AB_2656418, AB_2656419, AB_2656412

References for CD223 Antibody, anti-mouse, REAfinity™

Publications

  1. Baixeras, E. et al. (1992) Characterization of the lymphocyte activation gene 3-encoded protein. A new ligand for human leukocyte antigen class II antigens. J. Exp. Med. 176(2): 327-337
  2. Workman, C. J. et al. (2002) Phenotypic analysis of the murine CD4-related glycoprotein, CD223 (LAG-3). Eur. J. Immunol. 32(8): 2255-2263
  3. Workman, C. J. et al. (2002) Cutting edge: Molecular analysis of the negative regulatory function of lymphocyte activation gene-3. J. Immunol. 169(10): 5392-5395
  4. Kisielow, M. et al. (2005) Expression of lymphocyte activation gene 3 (LAG-3) on B cells is induced by T cells. Eur. J. Immunol. 35(7): 2081-2088

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