Clone:
REA290
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
IL17RA, CANDF5, CDw217, IL-17RA, IL17R, hIL-17R

Extended validation for CD217 (IL-17RA) Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD217 (IL-17RA) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD217 (IL-17RA)-PE (REA290, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD217 (IL-17RA) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD217 (IL-17RA)-PE (REA290, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD217 (IL-17RA) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD217 (IL-17RA)-PE (REA290, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD117 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD117-PE, clone (REA290). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD117 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD117-PE, clone (REA290). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD217 (IL-17RA) Antibody, anti-human, REAfinity™

Overview

Clone REA290 recognizes the human CD217 antigen, a single-pass type I membrane protein which is also known as interleukin-17 receptor A (IL-17RA). CD217 is a ubiquitously expressed receptor and subsequently shown to bind interleukin 17 (IL-17) with high affinity. IL-17 is an inflammatory cytokine that acts as a potent mediator in delayed-type reactions by increasing chemokine production in various tissues to recruit monocytes and neutrophils to the site of inflammation. It also plays critical roles in the pathogenesis of multiple autoimmune diseases. CD217 forms heterodimeric complexes with several other members of its receptor family, including IL-17RC for IL-17A and IL-17F signaling and IL-17RC for IL-17E (IL-25) signaling.
Additional information: Clone REA290 displays negligible binding to Fc receptors

Alternative names

IL17RA, CANDF5, CDw217, IL-17RA, IL17R, hIL-17R

Detailed product information

Technical specifications

CloneREA290
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD217 (IL-17RA)
Alternative names of antigenIL17RA, CANDF5, CDw217, IL-17RA, IL17R, hIL-17R
Molecular mass of antigen [kDa]92
Distribution of antigenmonocytes, neutrophils
Entrez Gene ID23765
RRIDAB_2819389, AB_2656335, AB_2656336, AB_2656341, AB_2656342, AB_2656343, AB_2656344, AB_2904839, AB_2904838, AB_2819391

Resources for CD217 (IL-17RA) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD217 (IL-17RA) Antibody, anti-human, REAfinity™

Publications

  1. Ding, Y. et al. (2013) IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells. J Immunol 191(4): 1614-1624
  2. Toy, D. et al. (2006) Cutting edge: interleukin 17 signals through a heteromeric receptor complex. J Immunol 177(1): 36-39
  3. Yao, Z. et al. (1997) Molecular characterization of the human interleukin (IL)-17 receptor. Cytokine 9(11): 794-800

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