Clone:
REA337
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Procr, CCCA, CCD41, EPCR

Extended validation for CD201 (EPCR) Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD201 (EPCR) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD201 (EPCR)-PE (REA337, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD201 (EPCR) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD201 (EPCR)-PE (REA337, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD201 (EPCR) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD201 (EPCR)-PE (REA337, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD201 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD201-PE, clone (REA337). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD201 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD201-PE, clone (REA337). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD201 (EPCR) Antibody, anti-human, REAfinity™

Overview

Clone REA337 recognizes the human CD201 antigen, a single-pass type I membrane protein which is also known as endothelial protein C receptor (EPCR). CD201 is strongly expressed in the endothelial cells of arteries and veins in heart and lung, and less intensely in capillaries in the lung and skin. It is also expressed on a subset of neutrophils, monocytes, and several tumor cells. The major role of CD201 is anticoagulation. CD201 binds to protein C with high affinity and augments the activation of protein C by the thrombin-thrombomodulin complex. Another important functional aspect of CD201 is its role in many pathophysiologic processes, such as inflammation responses to infection, trauma, hematopoiesis, and autoimmune response. Mutations in the CD201 gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.
Additional information: Clone REA337 displays negligible binding to Fc receptors.

Alternative names

Procr, CCCA, CCD41, EPCR

Detailed product information

Technical specifications

CloneREA337
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD201 (EPCR)
Alternative names of antigenProcr, CCCA, CCD41, EPCR
Molecular mass of antigen [kDa]25
Distribution of antigenendothelial cells, monocytes, heart, lung, skin
Entrez Gene ID10544
RRIDAB_2857581, AB_2784057, AB_2784056, AB_2801996, AB_2801975, AB_2889501, AB_2889500, AB_2656138, AB_2656139, AB_2922172, AB_2922148, AB_2857584

Resources for CD201 (EPCR) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD201 (EPCR) Antibody, anti-human, REAfinity™

Publications

  1. Iwasaki, H. et al. (2010) Endothelial protein C receptor-expressing hematopoietic stem cells reside in the perisinusoidal niche in fetal liver. Blood 116(4): 544-553
  2. Sturn, D. H. et al. (2003) Expression and function of the endothelial protein C receptor in human neutrophils. Blood 102(4): 1499-1505
  3. Willcox, C. R. et al. (2012) Cytomegalovirus and tumor stress surveillance by binding of a human γδ T cell antigen receptor to endothelial protein C receptor. Nat Immunol 13(9): 872-879
  4. Fukudome, K. et al. (1994) Identification, cloning, and regulation of a novel endothelial cell protein C/activated protein C receptor. J. Biol. Chem. 269(42): 26486-26491

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