Clone:
REA458
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MC
Alternative names:
CXCR6, BONZO, STRL33, TYMSTR

Extended validation for CD186 (CXCR6) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA458
13B1E5++
K041E5++
Cells were incubated with an excess of purified unconjugated CD186 (CXCR6) (REA458) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD186 (CXCR6). Human peripheral blood mononuclear cells (PBMCs) were stained with CD186 (CXCR6) antibodies and with a suitable counterstaining. As a control, CD186 (CXCR6) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD186 (CXCR6). Human peripheral blood mononuclear cells (PBMCs) were stained with CD186 (CXCR6) antibodies and with a suitable counterstaining. As a control, CD186 (CXCR6) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD186 (CXCR6). Human peripheral blood mononuclear cells (PBMCs) were stained with CD186 (CXCR6) antibodies and with a suitable counterstaining. As a control, CD186 (CXCR6) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD186 (CXCR6). Human peripheral blood mononuclear cells (PBMCs) were stained with CD186 (CXCR6) antibodies and with a suitable counterstaining. As a control, CD186 (CXCR6) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD186 (CXCR6) (REA458). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD186 (CXCR6) (REA458). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD186 (CXCR6) (REA458). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD186 (CXCR6) Antibody, anti-human, REAfinity™

Overview

Clone REA458 recognizes the human CD186 antigen, a multi-pass membrane protein which is also known as C-X-C chemokine receptor type 6 (CXCR6), STRL33, or bonzo. CD186 is expressed on a subset of type 1 polarized peripheral blood CD4
+
and CD8
+
T cells, γδ T cells, natural killer T cells, natural killer cells, monocytes, and B cells. It is induced in activated peripheral blood lymphocytes. The ligand of CD186 is the chemokine CXCL16. CD186 is used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1.
Additional information: Clone REA458 displays negligible binding to Fc receptors.

Alternative names

CXCR6, BONZO, STRL33, TYMSTR

Detailed product information

Technical specifications

CloneREA458
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD186 (CXCR6)
Alternative names of antigenCXCR6, BONZO, STRL33, TYMSTR
Molecular mass of antigen [kDa]39
Distribution of antigenT cells, NK cells, monocytes, B cells, NKT cells
Entrez Gene ID10663
RRIDAB_2819494, AB_2801897, AB_2655802, AB_2655803, AB_2819502

Resources for CD186 (CXCR6) Antibody, anti-human, REAfinity™

References for CD186 (CXCR6) Antibody, anti-human, REAfinity™

Publications

  1. Wehr, A. et al. (2013) Chemokine receptor CXCR6-dependent hepatic NK T Cell accumulation promotes inflammation and liver fibrosis. J. Immunol. 190(10): 5226-5236
  2. Deng, H. K. et al. (1997) Expression cloning of new receptors used by simian and human immunodeficiency viruses. Nature 388(6639): 296-300
  3. Deng, L. et al. (2010) CXCR6/CXCL16 functions as a regulator in metastasis and progression of cancer. Biochim. Biophys. Acta 1806(1): 42-49

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