Clone:
REA227
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
NK1.1, NKR-P1C, Ly-55

Extended validation for CD161 Antibody, anti-rat, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA227
10/78++
3.2.3++
Cells were incubated with an excess of purified unconjugated CD161 (REA227) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD161. Wistar-rat cells (4-5w) were stained with CD161 antibodies and with a suitable counterstaining. As a control, CD161 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD161. Wistar-rat cells (4-5w) were stained with CD161 antibodies and with a suitable counterstaining. As a control, CD161 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD161. Wistar-rat cells (4-5w) were stained with CD161 antibodies and with a suitable counterstaining. As a control, CD161 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD161. Wistar-rat cells (4-5w) were stained with CD161 antibodies and with a suitable counterstaining. As a control, CD161 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD161. Wistar-rat cells (4-5w) were stained with CD161 antibodies and with a suitable counterstaining. As a control, CD161 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD161 (REA227). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD161 (REA227). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD161 (REA227). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD161 Antibody, anti-rat, REAfinity™

Overview

Clone REA227 recognizes rat CD161, a C-type lectin membrane glycoprotein also known as NKR-P1. CD161 is expressed as an 80 kDa disulphide-linked homodimer and is found on most NK cells, T cell subsets, and many dendritic cells. In peripheral blood, CD161 is preferentially expressed on T cells of memory phenotype, but it can also be found on subsets of thymocytes and fetal liver T cells. CD161 has been implicated in triggering NK-mediated cytotoxicity, contributing to target cell recognition by NK cells.
Additional information: Clone REA227 displays negligible binding to Fc receptors.

Alternative names

NK1.1, NKR-P1C, Ly-55

Detailed product information

Technical specifications

CloneREA227
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesrat
AntigenCD161
Alternative names of antigenNK1.1, NKR-P1C, Ly-55
Molecular mass of antigen [kDa]25
Distribution of antigenNK cells, granulocytes, dendritic cells
Entrez Gene ID25192, 362443, 678513, 689817, 683758
RRIDAB_2655443, AB_2655444, AB_2655445, AB_2655446, AB_2655447, AB_2655448, AB_2655449, AB_2655450, AB_2655451, AB_2655442

Resources for CD161 Antibody, anti-rat, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD161 Antibody, anti-rat, REAfinity™

Publications

  1. Giorda, R. et al. (1990) NKR-P1, a signal transduction molecule on natural killer cells. Science 249(4974): 1298-1300
  2. Li, J. et al. (2003) Expression cloning and function of the rat NK activating and inhibitory receptors NKR-P1A and -P1B. Int. Immunol. 15(3): 411-416

Related products for
CD161 Antibody, anti-rat, REAfinity™

2 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?