Clone:
REA423
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRIII

Extended validation for CD16 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA423
3G8++
eBioCB16 (CB16)++
B73.1++
CLB-Fc-gran1/5D2++
Cells were incubated with an excess of purified unconjugated CD16 (REA423) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD16. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD16 antibodies and with a suitable counterstaining. As a control, CD16 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD16 antibodies and with a suitable counterstaining. As a control, CD16 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD16 antibodies and with a suitable counterstaining. As a control, CD16 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD16 antibodies and with a suitable counterstaining. As a control, CD16 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD16 antibodies and with a suitable counterstaining. As a control, CD16 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD16. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD16 antibodies and with a suitable counterstaining. As a control, CD16 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD16 (REA423). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD16 (REA423). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD16 (REA423). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD16 Antibody, anti-human, REAfinity™

Overview

Clone REA423 recognizes the human CD16 antigen, a single-pass type I membrane protein which is also known as low affinity immunoglobulin gamma Fc region receptor III (FcRIII). CD16 is a 50–80 kDa glycoprotein that is expressed in two different isoforms. The transmembrane form is found on human NK cells, macrophages, and mast cells, while the glycosylphosphatidylinositol (GPI)-linked form is present on neutrophils. The human CD16 antigen is a low-affinity receptor for aggregated IgG. The transmembrane form plays a role in signal transduction, NK cell activation, and antibody-dependent cellular cytotoxicity. Clone REA423 recognizes both the extracellular domain of the transmembrane form as well as the GPI-linked form of the human CD16 antigen. CD16 is expressed on the majority of rhesus monkey NK cells and on a subset of monocytes but not on granulocytes.
Additional information: Clone REA423 displays negligible binding to Fc receptors.

Alternative names

FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRIII

Detailed product information

Technical specifications

CloneREA423
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
, capuchin monkey,
chimpanzee (
Pan troglodytes
)
,
common marmoset (
Callithrix jacchus
)
,
cotton-top tamarin (
Saguinus oedipus
)
,
olive baboon (
Papio anubis
)
,
pigtail monkey (
Macaca nemestrina
)
,
squirrel monkey (
Saimiri sciureus
)
AntigenCD16
Alternative names of antigenFCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRIII
Molecular mass of antigen [kDa]48(sum of molecular weights of subunits)
Distribution of antigenNK cells, monocytes, granulocytes
Entrez Gene ID2214, 2215
RRIDAB_2726150, AB_2726428, AB_2726151, AB_2726426, AB_2726149, AB_2726431, AB_2726154, AB_2726432, AB_2726155, AB_2726429, AB_2726152, AB_2733100, AB_2733101, AB_2726430, AB_2726153, AB_2751177, AB_2751110, AB_2751814, AB_2751758, AB_2751968, AB_2751967, AB_2655423, AB_2726427

Reviews for CD16 Antibody, anti-human, REAfinity™

CD16 Expression for the Analysis of Human Peripheral Blood Neutrophils

  • 1
  • 2
  • 3
  • 4
  • 5

CD16-FITC, human (130-113-392)

Specifc and bright signal without elaborate optimisation! A great product!

Staining Peripheral Blood with Anti-CD16 PE

  • 1
  • 2
  • 3
  • 4
  • 5

CD16-PE, human (130-113-393)

Works well. Reproducible results.

Staining Peripheral Blood with Anti-CD16 PE

  • 1
  • 2
  • 3
  • 4
  • 5

CD16-PE, human (130-113-955)

Works well. Reproducible results.

References for CD16 Antibody, anti-human, REAfinity™

Publications

  1. Romee, R. et al. (2013) NK cell CD16 surface expression and function is regulated by a disintegrin and metalloprotease-17 (ADAM17). Blood 121(18): 3599-3608
  2. Ravetch, J. V. et al. (1989) Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions. J. Exp. Med. 170(2): 481-497
  3. Ziegler-Heitbrock, L. (2007)
    The CD14
    +
    CD16
    +
    blood monocytes: their role in infection and inflammation.
    J. Leukoc. Biol. 584(592): 584-592
  4. Croci, S. et al. (2018) Higher frequencies of lymphocytes expressing the natural killer group 2D receptor in patients with Behçet disease. Front Immunol 9: 2157
  5. Veluchamy, J. et al. (2017)
    In vivo
    efficacy of umbilical cord blood stem cell-derived NK cells in the treatment of metastatic colorectal cancer.
    Front Immunol 8: 87

Related products for
CD16 Antibody, anti-human, REAfinity™

3 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?