Name: CD16 antibodies
Availability: InStock

Product data and images for CD16 antibodies

Figures

Figure 1

Human EDTA-treated whole blood samples were stained with CD16 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. For analysis, white blood cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence.

View details

Figure 1

Human EDTA-treated whole blood samples were stained with CD16 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. For analysis, white blood cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence.

View details

Figure 1

Human EDTA-treated whole blood samples were stained with CD16 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. For analysis, white blood cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence.

View details

Figure 1

Human EDTA-treated whole blood samples were stained with CD16 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. For analysis, white blood cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence.

Specifications for CD16 antibodies

Overview

Clone REA423 recognizes the human CD16 antigen, a single-pass type I membrane protein which is also known as low affinity immunoglobulin gamma Fc region receptor III (FcRIII). CD16 is a 50–80 kDa glycoprotein that is expressed in two different isoforms. The transmembrane form is found on human NK cells, macrophages, and mast cells, while the glycosylphosphatidylinositol (GPI)-linked form is present on neutrophils. The human CD16 antigen is a low-affinity receptor for aggregated IgG. The transmembrane form plays a role in signal transduction, NK cell activation, and antibody-dependent cellular cytotoxicity. Clone REA423 recognizes both the extracellular domain of the transmembrane form as well as the GPI-linked form of the human CD16 antigen. CD16 is expressed on the majority of rhesus monkey NK cells and on a subset of monocytes but not on granulocytes.

Additional information: Clone REA423 displays negligible binding to Fc receptors.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.

Expression of CD16 may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Alternative names

FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRII

Detailed product information

Technical specifications

Clone	REA423
Isotype	recombinant human IgG1
Type of antibody	Recombinant antibodies
Species	human
Antigen	CD16
Alternative names of antigen	FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRII
Molecular mass of antigen [kDa]	48
Distribution of antigen	NK cells, monocytes, granulocytes, dendritic cells, macrophages, mast cells, neutrophils

Resources for CD16 antibodies

Documents and Protocols

Brochures/posters

CE IVD reagents - Support for GxP processes in cell manufacturing

Certificates

Please follow this

link

to download the Certificate of Conformity (CoC) by lot number.

References for CD16 antibodies

Publications

Romee, R. et al. (2013) NK cell CD16 surface expression and function is regulated by a disintegrin and metalloprotease-17 (ADAM17). Blood 121(18): 3599-3608
Ravetch, J. V. et al. (1989) Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions. J. Exp. Med. 170(2): 481-497
Ziegler-Heitbrock, L. (2007)
The CD14
⁺
CD16
⁺
blood monocytes: their role in infection and inflammation.
J. Leukoc. Biol. 584(592): 584-592
Wood BL et al. (2007) 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia. Cytometry B Clin. Cytom. 72: 14-22
Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition

Data and images

Data and images for CD16 antibodies

Figures

Figure 1

Human EDTA-treated whole blood samples were stained with CD16 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. For analysis, white blood cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence.

View details

Figure 1

Human EDTA-treated whole blood samples were stained with CD16 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. For analysis, white blood cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence.

View details

Figure 1

Human EDTA-treated whole blood samples were stained with CD16 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. For analysis, white blood cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence.

View details

Figure 1

Human EDTA-treated whole blood samples were stained with CD16 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. For analysis, white blood cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence.

Specifications

Specifications for CD16 antibodies

Overview

Additional information: Clone REA423 displays negligible binding to Fc receptors.

Applications

Expression of CD16 may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Alternative names

FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRII

Detailed product information

Technical specifications

Clone	REA423
Isotype	recombinant human IgG1
Type of antibody	Recombinant antibodies
Species	human
Antigen	CD16
Alternative names of antigen	FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRII
Molecular mass of antigen [kDa]	48
Distribution of antigen	NK cells, monocytes, granulocytes, dendritic cells, macrophages, mast cells, neutrophils

Resources

Resources for CD16 antibodies

Documents and Protocols

Brochures/posters

CE IVD reagents - Support for GxP processes in cell manufacturing

Certificates

Please follow this

link

to download the Certificate of Conformity (CoC) by lot number.

References

References for CD16 antibodies

Publications

Romee, R. et al. (2013) NK cell CD16 surface expression and function is regulated by a disintegrin and metalloprotease-17 (ADAM17). Blood 121(18): 3599-3608
Ravetch, J. V. et al. (1989) Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions. J. Exp. Med. 170(2): 481-497
Ziegler-Heitbrock, L. (2007)
The CD14
⁺
CD16
⁺
blood monocytes: their role in infection and inflammation.
J. Leukoc. Biol. 584(592): 584-592
Wood BL et al. (2007) 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia. Cytometry B Clin. Cytom. 72: 14-22
Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition

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CD16 antibodies

CD16 antibodies

Product data and images for CD16 antibodies

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for CD16 antibodies

Overview

Applications

Alternative names

Detailed product information

Technical specifications

Resources for CD16 antibodies

Documents and Protocols

CE IVD reagents - Support for GxP processes in cell manufacturing

Certificates

References for CD16 antibodies

Publications

Data and images

Data and images for CD16 antibodies

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Specifications

Specifications for CD16 antibodies

Overview

Applications

Alternative names

Detailed product information

Technical specifications

Resources

Resources for CD16 antibodies

Documents and Protocols

CE IVD reagents - Support for GxP processes in cell manufacturing

Certificates

References

References for CD16 antibodies

Publications

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