Clone:
REA309
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
ADAM10, AD10, AD18, HsT18717, MADM, RAK, kuz

Extended validation for CD156c (ADAM10) Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD156c (ADAM10) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD156c (ADAM10)-PE (REA309, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD156c (ADAM10) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD156c (ADAM10)-PE (REA309, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD156c (ADAM10) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD156c (ADAM10)-PE (REA309, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD156c knockout cells. Wild type (red) and knockout cells (blue) were stained with CD156c-PE, clone (REA309). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD156c knockout cells. Wild type (red) and knockout cells (blue) were stained with CD156c-PE, clone (REA309). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD156c (ADAM10) Antibody, anti-human, REAfinity™

Overview

Clone REA309 recognizes the human CD156c antigen, a single-pass type I membrane protein which is also known as disintegrin or metalloproteinase domain-containing protein 10 (ADAM10). CD156c belongs to the family of disintegrin and metalloproteinases (ADAMs) and is expressed ubiquitously on hematopoietic and non-hematopoietic cells. ADAMs have emerged as the major proteinase family that mediates proteolytic ectodomain release, a process known as shedding. This shedding process has been recognised as a key mechanism for regulating the function of a diversity of cell surface proteins. Dysregulation of ectodomain shedding is associated with autoimmune and cardiovascular diseases, neurodegeneration, infection, inflammation, and cancer. CD156c has been studied in the context of ectodomain shedding and has been demonstrated as key molecule in most of the shedding events in cancer and neurodegenerative disorders.
Additional information: Clone REA309 displays negligible binding to Fc receptors.

Alternative names

ADAM10, AD10, AD18, HsT18717, MADM, RAK, kuz

Detailed product information

Technical specifications

CloneREA309
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD156c (ADAM10)
Alternative names of antigenADAM10, AD10, AD18, HsT18717, MADM, RAK, kuz
Molecular mass of antigen [kDa]59
Distribution of antigenother
Entrez Gene ID102
RRIDAB_2655302, AB_2655303, AB_2655304, AB_2655305, AB_2655306, AB_2655307, AB_2655308, AB_2655301

References for CD156c (ADAM10) Antibody, anti-human, REAfinity™

Publications

  1. Weber, C. et al. (2013) Regulation of adult hematopoiesis by the a disintegrin and metalloproteinase 10 (ADAM10). Biochem. Biophys. Res. Commun. 442(3-4): 234-241
  2. Moss, M. L. and Bartsch, J. W. (2004) Therapeutic benefits from targeting of ADAM family members. Biochem. J. 43(23): 7227-7235
  3. Rosendahl, M. S. et al. (1997) Identification and characterization of a pro-tumor necrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases. J. Biol. Chem. 272(39): 24588-24593

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