Clone:
REA1081
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
PVR, PVS, NECL-5, TAGE4

Extended validation for CD155 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1081
SKII.4-
TX24-
2H7CD155-
PV.404.19++
Cells were incubated with an excess of purified unconjugated CD155 (REA1081) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD155 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD155-PE (REA1081, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD155 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD155-PE (REA1081, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD155 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD155-PE (REA1081, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD155 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD155-PE, clone (REA1081). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD155 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD155-PE, clone (REA1081). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD155 Antibody, anti-human, REAfinity™

Overview

Clone REA1081 recognizes the human CD155 antigen, a single-pass type I membrane protein also known as poliovirus receptor (PVR) or nectin-like protein 5 (NECL-5). CD155 is composed of two Ig-like C2-type domains and one Ig-like V-type domain that is necessary for poliovirus binding and uptake. Four different isotypes (α to δ) exist, originated from alternate splicing of the CD155 mRNA. CD155 is a broadly expressed receptor which is found on, for example, monocytes, a T cell subset, and neurons of the central nervous system. CD155 is also expressed on some CD34
+
progenitor cells, particulary on progenitors committed in myeloid differentiation. It can interact with several ligands, such as CD226, TIGIT, CD96, vitronectin, integrin αvβ3, and PDGFR. The functions of CD155 include roles in cell adhesion, neural differentiation, and NK cell effector functions.
Additional information: Clone REA1081 displays negligible binding to Fc receptors.

Alternative names

PVR, PVS, NECL-5, TAGE4

Detailed product information

Technical specifications

CloneREA1081
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD155
Alternative names of antigenPVR, PVS, NECL-5, TAGE4
Molecular mass of antigen [kDa]43
Distribution of antigenmonocytes, T cells, CNS cells
Entrez Gene ID5817
RRIDAB_2733617, AB_2733769, AB_2733770, AB_2733343, AB_2733344, AB_2732960, AB_2732961, AB_2733202, AB_2733203, AB_2733096, AB_2733097, AB_2734004, AB_2734005, AB_2921800, AB_2921794, AB_2733616

Resources for CD155 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD155 Antibody, anti-human, REAfinity™

Publications

  1. Mendelsohn, C. L. et al. (1989) Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily. Cell 56(5): 855 -865
  2. Lozano, E. et al. (2013) The CD226/CD155 interaction regulates the proinflammatory (Tʜ1/Tʜ17)/anti-inflammatory (Tʜ2) balance in humans. J Immunol 191(7): 3673-3680

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