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Splenocytes from a KLH-immunized mouse were restimulated in vitro with or without KLH, CD28, and CD40 antibodies for six hours. Cells were harvested and either unstained or stained with CD154 antibodies and CD4 antibodies, and analyzed by flow cytometry. A lymphocyte gate based on forward and side scatter properties was activated. Dead cells and B cells were excluded according to PI and CD45R/B220-PerCP™ staining.
Splenocytes from a KLH-immunized mouse were restimulated in vitro with or without KLH, CD28, and CD40 antibodies for six hours. Cells were harvested and either unstained or stained with CD154 antibodies and CD4 antibodies, and analyzed by flow cytometry. A lymphocyte gate based on forward and side scatter properties was activated. Dead cells and B cells were excluded according to PI and CD45R/B220-PerCP™ staining.
Clone | MR1 |
---|---|
Clonality | monoclonal |
Isotype | hamster IgG3κ |
Host | hamster |
Type of antibody | Primary antibodies |
Species | mouse |
Antigen | CD154 |
Alternative names of antigen | CD40LG, CD40L, HIGM1, IGM, IMD3, Ly-62, T-BAM, TRAP, TNFSF5, gp39 |
Molecular mass of antigen [kDa] | 29 |
Distribution of antigen | dendritic cells, macrophages, mast cells, monocytes, NK cells, T cells, lymphocytes, basophils |
Entrez Gene ID | 21947 |
RRID | AB_2661125, AB_2661126, AB_2661127, AB_2661128, AB_2661124 |
Clone | MR1 |
---|---|
Clonality | monoclonal |
Isotype | hamster IgG3κ |
Host | hamster |
Type of antibody | Primary antibodies |
Species | mouse |
Antigen | CD154 |
Alternative names of antigen | CD40LG, CD40L, HIGM1, IGM, IMD3, Ly-62, T-BAM, TRAP, TNFSF5, gp39 |
Molecular mass of antigen [kDa] | 29 |
Distribution of antigen | dendritic cells, macrophages, mast cells, monocytes, NK cells, T cells, lymphocytes, basophils |
Entrez Gene ID | 21947 |
RRID | AB_2661125, AB_2661126, AB_2661127, AB_2661128, AB_2661124 |
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