Clone:
REA1003
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
CTLA4, ALPS5, CD, CELIAC3, CTLA-4, GRD4, GSE, IDDM12

Extended validation for CD152 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1003
L3D10++
14D3-
BNI3++
Cells were incubated with an excess of purified unconjugated CD152 (REA1003) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD152. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with CD152 antibodies. As a control, CD152 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD152. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with CD152 antibodies. As a control, CD152 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD152. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with CD152 antibodies. As a control, CD152 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD152. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with CD152 antibodies. As a control, CD152 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD152. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with CD152 antibodies. As a control, CD152 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD152 Antibody, anti-human, REAfinity™

Overview

Clone REA1003 recognizes the human CD152, an integral membrane protein of the immunoglobin superfamily which is also called CTLA-4. CD152 is a negative regulator of T cell activation. It is transiently expressed on activated CD28
+
T cells and is a ligand for CD80 and CD86. CD4
+
FoxP3
+
regulatory T cells are reported to constitutively express CD152. CD152 may also be detected on B cells when cultured with activated T cells.
Additional information: Clone REA1003 displays negligible binding to Fc receptor.

Alternative names

CTLA4, ALPS5, CD, CELIAC3, CTLA-4, GRD4, GSE, IDDM12

Detailed product information

Technical specifications

CloneREA1003
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
cynomolgus monkey (
Macaca fascicularis
)
,
rhesus monkey (
Macaca mulatta
)
,
chimpanzee (
Pan troglodytes
)
,
olive baboon (
Papio anubis
)
AntigenCD152
Alternative names of antigenCTLA4, ALPS5, CD, CELIAC3, CTLA-4, GRD4, GSE, IDDM12
Molecular mass of antigen [kDa]21
Distribution of antigenB cells, T cells, regulatory T cells
Entrez Gene ID1493
RRIDAB_2727694, AB_2727768, AB_2727695, AB_2727769, AB_2727696, AB_2728075, AB_2728068, AB_2727770, AB_2727697, AB_2727767

References for CD152 Antibody, anti-human, REAfinity™

Publications

  1. Kuiper, H. M. et al. (1995) Activated T cells can induce high levels of CTLA-4 expression on B cells. J. Immunol. 155(4): 1776-1783
  2. Castan, J. et al. (1997) Accumulation of CTLA-4 expressing T lymphocytes in the germinal centres of human lymphoid tissues. Immunology 90(2): 265-271
  3. Schlotmann, T. et al. (2000) CD alphabeta T lymphocytes express high levels of the T lymphocyte antigen CTLA-4 (CD152) in acute malaria. J. Infect. Dis. 182(1): 367-370
  4. Sansom, D. M. and Walker, L. S. (2006) The role of CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4) in regulatory T-cell biology. Immunol. Rev. 212: 131-148

Related products for
CD152 Antibody, anti-human, REAfinity™

2 products available