Clone:
REAL507
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
MCAM, 1-gicerin, CD149, MUC18, S-endo, s-gicerin, Endo-CAM, Mel-CAM

Specifications for CD146 (LSEC) Antibody, anti-mouse, REAdye_lease™

Overview

Clone REAL507 is an antibody fragment derived from the full CD146 (LSEC) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL507 recognizes the mouse CD146 antigen, which is expressed on mouse endothelial cells, including liver sinusoidal endothelial cells (LSECs), smooth muscle cells, and the basal membrane. LSECs are microvascular endothelial cells lining the hepatic sinusoidal wall. Their strategic positioning favors a tight interaction with lymphocytes migrating through the liver. They possess a high capacity for antigen uptake and processing. However, in contrast to professional antigen-presenting cells, e.g. dendritic cells, they express only low levels of costimulatory molecules.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

MCAM, 1-gicerin, CD149, MUC18, S-endo, s-gicerin, Endo-CAM, Mel-CAM

Detailed product information

Technical specifications

CloneREAL507
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD146 (LSEC)
Alternative names of antigenMCAM, 1-gicerin, CD149, MUC18, S-endo, s-gicerin, Endo-CAM, Mel-CAM
Distribution of antigenendothelial cells, smooth muscle

References for CD146 (LSEC) Antibody, anti-mouse, REAdye_lease™

Publications

  1. Harder, R. et al. (1991) Dissection of murine lymphocyte-endothelial cell interaction mechanisms by SV-40-transformed mouse endothelial cell lines: novel mechanisms mediating basal binding, and alpha 4-integrin-dependent cytokine-induced adhesion. Exp. Cell Res. 197: 259-267
  2. Diehl, L. et al. (2008)
    Tolerogenic maturation of liver sinusoidal endothelial cells promotes B7-homolog 1-dependent CD8
    +
    T cell tolerance.
    Hepatology 47: 296-305

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