CD144 (VE-Cadherin) MicroBeads have been developed for the positive selection or depletion of CD144
+
endothelial cells from differentiated ES or iPS cell cultures.

Data and images for CD144 (VE-Cadherin) MicroBeads, human

Figures

Figure 1

Human ES cells were differentiated into endothelial cells using the protocol of Patsch et al.. CD144
+
cells were isolated from the heterogeneous mixture of differentiated cells using CD144 (VE-Cadherin) MicroBeads, an LS Column, and a MidiMACS Separator. For detection of endothelial cells, cells were fluorescently labeled with CD31-APC and analyzed using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
After separation
View details

Figure 1

Human ES cells were differentiated into endothelial cells using the protocol of Patsch et al.. CD144
+
cells were isolated from the heterogeneous mixture of differentiated cells using CD144 (VE-Cadherin) MicroBeads, an LS Column, and a MidiMACS Separator. For detection of endothelial cells, cells were fluorescently labeled with CD31-APC and analyzed using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Human ES cells were differentiated into endothelial cells using the protocol of Patsch et al.. CD144
+
cells were isolated from the heterogeneous mixture of differentiated cells using CD144 (VE-Cadherin) MicroBeads, an LS Column, and a MidiMACS Separator. For detection of endothelial cells, cells were fluorescently labeled with CD31-APC and analyzed using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD144 (VE-Cadherin) MicroBeads, human

Overview

CD144 (VE-Cadherin) MicroBeads have been developed for the positive selection or depletion of CD144
+
endothelial cells from differentiated ES or iPS cell cultures.

Detailed product information

Background information

CD144, also known as VE-Cadherin or Cadherin-5, is a member of the cadherin family of calcium-dependent adhesion molecules. The antigen is expressed on endothelial cells
in vivo
and
in vitro1,2
and has been used for the isolation of endothelial cells derived from embryonic stem cells and induced pluripotent stem cells
2,3,4
. It is known to be involved in homophilic cell interactions contributing to the cohesion and organization of the intercellular junctions of the vascular endothelium and thought to play a role in vascular permeability and remodeling. CD144 MicroBeads react with a calcium-independent epitope on CD144.

Columns

For positive selection: LS or autoMACS
®
Columns. For depletion: LD or autoMACS Columns.

References for CD144 (VE-Cadherin) MicroBeads, human

Publications

  1. Rufaihah, A. J. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 3: e72-e79
  2. Tatsumi, R. et al. (2011) Simple and highly efficient method for production of endothelial cells from human embryonic stem cells. Cell Transplant 20: 1423-1430
  3. Patsch, C. et al. (2015) Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells. Nat. Cell Biol. 17(8): 994-1003
  4. Thoma E.C. et al. (2016) Establishment of a translational endothelial cell model using directed differentiation of induced pluripotent stem cells from Cynomolgus monkey. Sci Rep 6: 35830

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