The CD140a (PDGFRα) MicroBead Kit was developed for the direct isolation or depletion of CD140a (PDGFRα) positive oligodendrocyte precursors from dissociated mouse brain tissue.

Data and images for CD140a (PDGFRα) MicroBead Kit, mouse

Figures

Figure 1

CD140a (PDGFRα)
+
cells were isolated from P2 CD-1 mouse brain tissue using the CD140a (PDGFRα) MicroBead Kit, the Neural Tissue Dissociation Kit (P), the gentleMACS Dissociator, a MiniMACS Separator, and an MS Column. Cells were fluorecently stained with Labeling Check Reagent-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
CD140a (PDGFRα)
-
cells
View details

Figure 1

CD140a (PDGFRα)
+
cells were isolated from P2 CD-1 mouse brain tissue using the CD140a (PDGFRα) MicroBead Kit, the Neural Tissue Dissociation Kit (P), the gentleMACS Dissociator, a MiniMACS Separator, and an MS Column. Cells were fluorecently stained with Labeling Check Reagent-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD140a (PDGFRα)
+
cells were isolated from P2 CD-1 mouse brain tissue using the CD140a (PDGFRα) MicroBead Kit, the Neural Tissue Dissociation Kit (P), the gentleMACS Dissociator, a MiniMACS Separator, and an MS Column. Cells were fluorecently stained with Labeling Check Reagent-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD140a (PDGFRα)
+
cells were isolated from P2 CD-1 mouse brain tissue using the CD140a (PDGFRα) MicroBead Kit, the Neural Tissue Dissociation Kit (P), the gentleMACS Dissociator, a MiniMACS Separator, and an MS Column. Cells were fluorecently stained with Labeling Check Reagent-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD140a (PDGFRα) MicroBead Kit, mouse

Overview

The CD140a (PDGFRα) MicroBead Kit was developed for the direct isolation or depletion of CD140a (PDGFRα) positive oligodendrocyte precursors from dissociated mouse brain tissue.

Detailed product information

Background information

CD140a (PDGFRα) MicroBeads (PDGFRα: platelet-derived growth factor receptor alpha) have been developed for the separation of mouse cells based on the expression of the CD140a (PDGFRα) antigen. CD140a (PDGFRα) has been reported to be broadly expressed in embryonic tissue, various malignancies and embryonic stem cell-derived cardiomyogenic cells.
1,2
Furthermore, CD140a (PDGFRα) is specifically expressed by oligodendrocyte precursor cells, which differentiate into myelinating oligodendrocytes.
3–5
CD140a (PDGFRα) MicroBeads were especially optimized for the separation of oligodendrocyte progenitor cells (OPCs) based on the expression of CD140a (PDGFRα). The isolation was tested particularly on dissociated postnatal CD1 mouse brain tissue derived from animals younger than postnatal day eight (P8). In principle, cell isolation is also possible from older mice, but purity of the positive fraction might be lower due to lower frequency of CD140a (PDGFRα) positive cells after tissue dissociation.
For optimal results, the Neural Tissue Dissociation Kit (P) is recommended prior to cell isolation.

Resources for CD140a (PDGFRα) MicroBead Kit, mouse

References for CD140a (PDGFRα) MicroBead Kit, mouse

Publications

  1. Heldin, C.H. and Westermark, B. (1999)
    Mechanism of action and
    in vivo
    role of platelet-derived growth factor.
    Physiol. Rev. 79: 1283-1316
  2. Takakura, N. et al. (1997) PDGFRα expression during mouse embryogenesis: immunolocalization analyzed by whole-mount immunohistostaining using the monoclonal antibody anti-mouse PDGFR alpha antibody APA5. J. Histochem. Cytochem. 45(6): 883-893
  3. Sim, F. J. et al. (2011) CD140a identifies a population of highly myelinogenic migration-competent and efficiently engrafting human oligodendrocyte progenitor cells. Nat. Biotechnol. 29: 934-941
  4. Nishiyama, A. et al. (1996) Co-localization of NG2 proteoglycan and PDGF alpha-receptor on O2A progenitor cells in the developing rat brain. J. Neurosci. Res. 43: 299-314
  5. Nishiyama, A. et al. (2009) Polydendrocytes (NG2 cells): multifunctional cells with lineage plasticity. Nat. Rev. Neurosci. 10: 9-22

Related products for
CD140a (PDGFRα) MicroBead Kit, mouse

3 products available