CD14 MicroBeads UltraPure can be used for the positive selection or depletion of human monocytes and macrophages from cord blood or PBMCs. They are particularly suited to work with low quality starting material, i. e., older buffy coats.

Data and images for CD14 MicroBeads UltraPure, human

Figures

Figure 1

CD14
+
cells were isolated from human PBMCs using CD14 MicroBeads UltraPure, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD14-PE and CD15-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
CD14
cells
CD14
+
cells
View details

Figure 1

CD14
+
cells were isolated from human PBMCs using CD14 MicroBeads UltraPure, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD14-PE and CD15-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD14
+
cells were isolated from human PBMCs using CD14 MicroBeads UltraPure, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD14-PE and CD15-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD14
+
cells were isolated from human PBMCs using CD14 MicroBeads UltraPure, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD14-PE and CD15-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD14 MicroBeads UltraPure, human

Overview

CD14 MicroBeads UltraPure can be used for the positive selection or depletion of human monocytes and macrophages from cord blood or PBMCs. They are particularly suited to work with low quality starting material, i. e., older buffy coats.

Detailed product information

Background information

The CD14 antigen belongs to the LPS receptor complex. Binding of antibody to CD14 does not trigger signal transduction since CD14 lacks a cytoplasmatic domain. CD14 is strongly expressed on most monocytes, macrophages, and weakly on neutrophils and some myeloid dendritic cells.

Downstream applications

CD14 MicroBeads UltraPure can be used for a variety of applications, for example:
  • Ex vivo differentiation into monocyte-derived dendritic cells (Mo-DCs) or monocyte-derived macrophages.
  • Studies on monocyte activation, differentiation, cytokine secretion etc.
  • Studies on compound or antigen uptake (e.g. phagocytosis) and antigen presentation by monocytes.

Columns

For positive selection: MS, LS, XS, autoMACS® Columns, or Multi-24 Column Block. For depletion: LD, CS, D, autoMACS Columns, or Multi-24 Column Block.

References for CD14 MicroBeads UltraPure, human

Publications

  1. Patel, V. I. et al. (2017) Transcriptional classification and functional characterization of human airway macrophage and dendritic cell subsets. J. Immunol. 198(3): 1183-1201
  2. Bakdash, G. et al. (2016)
    Expansion of a BDCA1
    +
    CD14
    +
    myeloid cell population in melanoma patients may attenuate the efficacy of dendritic cell vaccines.
    Cancer Res. 76(15): 4332-4346
  3. Sander, J. et al. (2017) Cellular differentiation of human monocytes is regulated by time-dependent interleukin-4 signaling and the transcriptional regulator NCOR2. Immunity 47(6): 1051-1066

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