Clone:
REA104
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
SDC1, Sstn, Synd, Synd1, SYN-1

Extended validation for CD138 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA104
281-2++
Cells were incubated with an excess of purified unconjugated CD138 (REA104) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD138. Balb/c bone marrow cells were stained with CD138 antibodies and with a suitable counterstaining. As a control, CD138 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD138. Balb/c bone marrow cells were stained with CD138 antibodies and with a suitable counterstaining. As a control, CD138 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD138. Balb/c bone marrow cells were stained with CD138 antibodies and with a suitable counterstaining. As a control, CD138 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD138. Balb/c bone marrow cells were stained with CD138 antibodies and with a suitable counterstaining. As a control, CD138 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD138 (REA104). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD138 (REA104). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD138 (REA104). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD138 Antibody, anti-mouse, REAfinity™

Overview

CD138, also known as syndecan-1, is a type I integral membrane protein belonging to the syndecan protein family which carries three to five heparan sulfate and chondroitin sulfate chains. CD138 is highly expressed on plasma cells and pre–B cells but not on mature peripheral B cells. It is also expressed on fibroblasts and epithelial cells. The expression of CD138 can be regulated by several cytokines and factors, e.g., thrombin, which is reported to increase the synthesis of CD138 molecules that exhibit high-affinity antithrombin-3 binding.
Additional information: Clone REA104 displays negligible binding to Fc receptors.

Alternative names

SDC1, Sstn, Synd, Synd1, SYN-1

Detailed product information

Technical specifications

CloneREA104
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD138
Alternative names of antigenSDC1, Sstn, Synd, Synd1, SYN-1
Molecular mass of antigen [kDa]30
Distribution of antigenB cells, endothelial cells, epithelial cells, neurons, plasma cells, breast, smooth muscle
Entrez Gene ID20969
RRIDAB_2752184, AB_2801979, AB_2655023, AB_2655024, AB_2655025, AB_2752204

References for CD138 Antibody, anti-mouse, REAfinity™

Publications

  1. Cizmeci-Smith, G. et al. (1997) Thrombin stimulates Syndecan-1 promotor activity and expression of a form of Syndecan-1 that binds antithrombin III in vascular smooth muscle cells. Arterioscler. Thromb. Vasc. Biol. 17: 2609 -2616
  2. Sanderson, R. D. et al. (1989) B lymphocytes express and lose syndecan at specific stages of differentiation. Cell Regul. 1(1): 27-35
  3. Jalkanen, M. et al. (1985) Heparan sulfate proteoglycans from mouse mammary epithelial cells: localization on the cell surface with a monoclonal antibody. J. Cell Biol. 101(3): 976-984
  4. Saunders, S. et al. (1989) Molecular cloning of syndecan, an integral membrane proteoglycan. J. Cell Biol. 108(4): 1547-1556
  5. Chilosi, M. et al. (1999) CD138/syndecan-1: a useful immunohistochemical marker of normal and neoplastic plasma cells on routine trephine bone marrow biopsies. Mod. Pathol. 12(12): 1101-1106

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