Clone:
REA621
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
OX-40, OX40

Extended validation for CD134 (OX40) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA621
Ber-ACT35++
L106-
443318 rIgG2a++
ACT35++
Cells were incubated with an excess of purified unconjugated CD134 (OX40) (REA621) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD134 (OX40). Human peripheral blood mononuclear cells (PBMCs) stimulated with CytoStim™ for 16h were stained with CD134 (OX40) antibodies and with a suitable counterstaining. As a control, CD134 (OX40) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD134 (OX40). Human peripheral blood mononuclear cells (PBMCs) stimulated with CytoStim™ for 16h were stained with CD134 (OX40) antibodies and with a suitable counterstaining. As a control, CD134 (OX40) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD134 (OX40). Human peripheral blood mononuclear cells (PBMCs) stimulated with CytoStim™ for 16h were stained with CD134 (OX40) antibodies and with a suitable counterstaining. As a control, CD134 (OX40) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD134 (OX40). Human peripheral blood mononuclear cells (PBMCs) stimulated with CytoStim™ for 16h were stained with CD134 (OX40) antibodies and with a suitable counterstaining. As a control, CD134 (OX40) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD134 (OX40). Human peripheral blood mononuclear cells (PBMCs) stimulated with CytoStim™ for 16h were stained with CD134 (OX40) antibodies and with a suitable counterstaining. As a control, CD134 (OX40) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD134 (OX40) (REA621). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD134 (OX40) (REA621). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD134 (OX40) (REA621). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD134 (OX40) Antibody, anti-human, REAfinity™

Overview

Clone REA621 recognizes the CD134 (OX40) antigen, a member of the tumor necrosis factor/nerve growth factor receptor (TNFR/NGFR) family. CD134 is a 50 kDa type I membrane glycoprotein expressed by activated T lymphocytes. The interaction of CD134 with OX40L has been implicated in T cell–dependent humoral response, regulation of primary T cell expansion, survival of T cells, size of the memory T cell pool, and regulation of tolerance in the CD4
+
T cell compartment. It is reported, that CD134 activates NF-κB through its interaction with adaptor proteins TRAF2 and TRAF5.
Additional information: Clone REA621 displays negligible binding to Fc receptors.

Alternative names

OX-40, OX40

Detailed product information

Technical specifications

CloneREA621
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD134 (OX40)
Alternative names of antigenOX-40, OX40
Molecular mass of antigen [kDa]27
Distribution of antigenB cells, endothelial cells, fibroblasts, T cells, lymphocytes
Entrez Gene ID7293
RRIDAB_2783943, AB_2783942, AB_2752198, AB_2752179, AB_2876990, AB_2876991, AB_2654939, AB_2654940

References for CD134 (OX40) Antibody, anti-human, REAfinity™

Publications

  1. Zaunders, J. J. et al. (2009)
    High levels of human antigen-specific CD4
    +
    T cells in peripheral blood revealed by stimulated coexpression of CD25 and CD134 (OX40).
    J. Immunol. 183: 2827-2836
  2. Croft, M. et al. (2009) The significance of OX40 and OX40L to T-cell biology and immune disease. Immunol. Rev. 229(1): 173-191
  3. Kawamata, S. et al. (1998) Activation of OX40 signal transduction pathways leads to tumor necrosis factor receptor-associated factor (TRAF) 2- and TRAF5-mediated NF-kappaB activation. J. Biol. Chem. 273(10): 5808-5814

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