The CD133 MicroBead Kit allows basic isolation or depletion of CD133
+
cells from various sources.
Note:
Optimized isolation kits with improved performance are available for the following tissues:
  • For hematopoietic or endothelial progenitor cells, choose the CD133 MicroBead Kit – Hematopoietic Tissue
  • For cancer or tissue stem cells the CD133 MicroBead Kit – Tumor Tissue is recommended

Data and images for CD133 MicroBead Kit, human

Figures

Figure 1

Isolation of CD133
+
cells from human PBMCs using the CD133 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator.
PBMCs before separation
Isolated CD133
+
cells
View details

Figure 1

Isolation of CD133
+
cells from human PBMCs using the CD133 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator.
View details

Figure 1

Isolation of CD133
+
cells from human PBMCs using the CD133 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator.

Specifications for CD133 MicroBead Kit, human

Overview

The CD133 MicroBead Kit allows basic isolation or depletion of CD133
+
cells from various sources.
Note:
Optimized isolation kits with improved performance are available for the following tissues:
  • For hematopoietic or endothelial progenitor cells, choose the CD133 MicroBead Kit – Hematopoietic Tissue
  • For cancer or tissue stem cells the CD133 MicroBead Kit – Tumor Tissue is recommended

Detailed product information

Background information

CD133, formerly known as AC133, recognizes epitope 1 of the CD133 antigen.
2,3
It is a marker that is frequently found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. In the hematopoietic system, CD133 is expressed on a small portion of CD34
cells
4
as well as on a subset of CD34
bright
stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood
5
. CD133 has also been found to be expressed on circulating endothelial progenitor cells,
6,7
fetal neural stem cells,
8,9
other tissue-specific stem cells, such as renal
10
, prostate
11
, and corneal
12
stem cells, cancer stem cells from tumor tissues, as well as ES and iPS cell-derived cells.
There is a growing interest in CD133-expressing stem cells from normal blood or bone marrow in regenerative medicine, for example, in the field of cardiovascular
13-17
, liver, or peripheral artery diseases
18-20
.
The CD133 MicroBeads included in the kit recognize epitope CD133/1. For quality control staining of isolated CD133
+
cells, the use of CD133/2 (293C3)-PE or -APC is recommended.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

References for CD133 MicroBead Kit, human

Publications

  1. Galic, Z. et al. (2006) T lineage differentiation from human embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A. 103: 11742-11747
  2. Yin, A. H. et al. (1997) AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 90: 5002-5012
  3. Piechaczek, C. (2001) CD133. J. Biol. Regul. Homeost. Agents 15: 101-102
  4. Gallacher, L. et al. (2000)
    Isolation and characterization of human CD34
    Lin
    and CD34
    +
    Lin
    hematopoietic stem cells using cell surface markers AC133 and CD7.
    Blood 95(ARVO Annual Meeting Abstract): 2813-2820
  5. Bühring, H. J. et al. (1999) Expression of novel surface antigens on early hematopoietic cells. Ann. N. Y. Acad. Sci. 872: 25-39
  6. Gehling, U. M. et al. (2000)
    In vitro
    differentiation of endothelial cells from AC133-positive progenitor cells.
    Blood 95: 3106-3112
  7. Peichev, M. et al. (2000)
    Expression of VEGFR-2 and AC133 by circulating human CD34
    +
    cells identifies a population of functional endothelial precursors.
    Blood 95: 952-958
  8. Uchida, N. et al. (2000) Direct isolation of human central nervous system stem cells. Proc. Natl. Acad. Sci. U.S.A. 97: 14720-14725
  9. Cummings, B. J. et al. (2005) Human neural stem cells differentiate and promote locometer recovery in spinal cord-injured mice. Proc. Natl. Acad. Sci. U.S.A. 102: 14069-14074
  10. Bussolati, B. et al. (2005) Isolation of renal progenitor cells from adult human kidney. Am. J. Pathol. 166: 545-555
  11. Richardson, G. et al. (2004) CD133, a novel marker for human prostatic epithelial stem cells. J. Cell. Sci. 117: 3539-3545
  12. Thill, M. et al. (2004)
    Identification of a population of CD133
    +
    precursor cells in the stroma of human cornea.
    Invest. Ophthalmol. Vis. Sci. 45: 3519
  13. Stamm, C. et al. (2003) Autologous bone-marrow stem-cell transplantation for myocardial regeneration. Lancet 361(9351): 45-46
  14. Stamm, C. et al. (2007)
    Intramyocardial delivery of CD133
    +
    bone marrow cells and coronary artery bypass grafting for chronic ischemic heart disease: safety and efficacy studies.
    J. Thorac. Cardiovasc. Surg. 133: 717-725
  15. Klein (2007) Eur. Cardiovasc. Dis. 1: 123-125
  16. Klein, H. M. et al. (2007)
    Intramyocardial implantation of CD133
    +
    stem cells improved cardiac function without bypass surgery.
    Heart Surg. Forum 10: E66-69
  17. Bartunek et al. (2005) Circulation 30: 178-183
  18. am Esch, J. S. 2nd et al. (2005)
    Portal application of autologous CD133
    +
    bone marrow cells to the liver: a novel concept to support hepatic regeneration.
    Stem Cells 23(4): 463-470
  19. Fürst, G. et al. (2007)
    Portal vein embolization and autologous CD133
    +
    bone marrow stem cells for liver regeneration: initial experience.
    J. Immunother. 243(1): 171-179
  20. Cañizo et al. (2007)
    Peripheral endothelial progenitor cells (CD133
    +
    ) for therapeutic vasculogenesis in a patient with critical limb ischemia. One year follow-up.
    Cytotherapy 9(1): 99-102

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