Clone:
REA614
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MC
Alternative names:
IL-7R, IL-7Rα, IL-17Ralpha, CDW127, ILRA

Extended validation for CD127 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA614
HIL-7R-M21++
eBioRDR5-
A019D5++
MB15-18C9++
Cells were incubated with an excess of purified unconjugated CD127 (REA614) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD127. Human peripheral blood mononuclear cells (PBMCs) were stained with CD127 antibodies and with a suitable counterstaining. As a control, CD127 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD127. Human peripheral blood mononuclear cells (PBMCs) were stained with CD127 antibodies and with a suitable counterstaining. As a control, CD127 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD127. Human peripheral blood mononuclear cells (PBMCs) were stained with CD127 antibodies and with a suitable counterstaining. As a control, CD127 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD127. Human peripheral blood mononuclear cells (PBMCs) were stained with CD127 antibodies and with a suitable counterstaining. As a control, CD127 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD127. Human peripheral blood mononuclear cells (PBMCs) were stained with CD127 antibodies and with a suitable counterstaining. As a control, CD127 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD127. Human peripheral blood mononuclear cells (PBMCs) were stained with CD127 antibodies and with a suitable counterstaining. As a control, CD127 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD127. Human peripheral blood mononuclear cells (PBMCs) were stained with CD127 antibodies and with a suitable counterstaining. As a control, CD127 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD127 (REA614). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD127 (REA614). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD127 (REA614). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD127 Antibody, anti-human, REAfinity™

Overview

Clone REA614 recognizes the CD127 antigen, which is the α-chain of the interleukin 7 (IL-7) receptor, a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc). CD127 can be identified on immature B cells through the early pre–B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation. On regulatory T cells CD127 is absent and its expression is inversely correlated with FoxP3 expression and suppressive function. CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.
Additional information: Clone REA614 displays negligible binding to Fc receptors.

Alternative names

IL-7R, IL-7Rα, IL-17Ralpha, CDW127, ILRA

Detailed product information

Technical specifications

CloneREA614
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD127
Alternative names of antigenIL-7R, IL-7Rα, IL-17Ralpha, CDW127, ILRA
Molecular mass of antigen [kDa]50
Distribution of antigenT cells
Entrez Gene ID3575
RRIDAB_2726163, AB_2733758, AB_2733759, AB_2726438, AB_2726161, AB_2733197, AB_2733198, AB_2726439, AB_2726162, AB_2783927, AB_2783926, AB_2811440, AB_2811433, AB_2801875, AB_2726440

Resources for CD127 Antibody, anti-human, REAfinity™

References for CD127 Antibody, anti-human, REAfinity™

Publications

  1. Fry, T. J. and Mackall, C. L. (2002) Interleukin-7: from bench to clinic. Blood 99: 3892-3904
  2. Seddiki, N. et al. (2006) Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J. Exp. Med. 203: 1693-1700
  3. Liu, W. et al. (2006)
    CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4
    +
    T reg cells.
    J. Exp. Med. 203: 1701-1711
  4. Sudo, T. et al. (1993) Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 90: 9125-9129
  5. Cupedo, T. et al. (2005) Development and activation of regulatory T cells in the human fetus. Eur. J. Immunol. 35: 383-390
  6. Armitage, R. J. et al. (1991) Expression of receptors for interleukin 4 and interleukin 7 on human T cells. Adv. Exp. Med. Biol. 292: 121-130

Related products for
CD127 Antibody, anti-human, REAfinity™

3 products available