Clone:
REA618
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, ICC, MC
Alternative names:
95, CR4, integrin αX, ITGAX, p150

Extended validation for CD11c Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA618
S-HCL-3++
3.9-
Bu15-
B-ly6-
MJ4-27G12-
Cells were incubated with an excess of purified unconjugated CD11c (REA618) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11c (REA618). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11c (REA618). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD11c (REA618). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD11c Antibody, anti-human, REAfinity™

Overview

Clone REA618 recognizes the human CD11c antigen, a 145–150 kDa type I transmembrane glycoprotein, which is also known as integrin αX or CR4. It is expressed on monocytes, macrophages, NK cells, granulocytes, myeloid dendritic cells (MDCs), and subsets of T and B cells. On myeloid dendritic cells, CD11c is highly expressed on type 1 myeloid dendritic cells (CD1c (BDCA-1)
+
CD123
low
MDC1s) and low on type 2 myeloid dendritic cells (CD1c (BDCA-1)
CD123
MDC2s). CD11c, also known as integrin integrin αX or CR4, has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.
Additional information: Clone REA618 displays negligible binding to Fc receptors.

Alternative names

95, CR4, integrin αX, ITGAX, p150

Detailed product information

Technical specifications

CloneREA618
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD11c
Alternative names of antigen95, CR4, integrin αX, ITGAX, p150
Molecular mass of antigen [kDa]126
Distribution of antigenB cells, T cells, NK cells, dendritic cells, granulocytes, monocytes, macrophages, leukemia cells
Entrez Gene ID3687
RRIDAB_2726189, AB_2726464, AB_2726187, AB_2726461, AB_2726184, AB_2726782, AB_2726698, AB_2726465, AB_2726188, AB_2726462, AB_2726185, AB_2726463, AB_2726186, AB_2783922, AB_2801877, AB_2726466

References for CD11c Antibody, anti-human, REAfinity™

Publications

  1. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
  2. Bendiss-Vermare, N. et al. (2001)
    Human thymus contains IFN-alpha-producing CD11c
    , myeloid CD11c
    +
    , and mature interdigitating dendritic cells.
    J. Clin. Invest. 107: 835-844
  3. Barclay, A. N. et al. (1997) In: The Leukocyte Antigen Facts Book, Academic Press, San Diego, CA. (2nd edition): 161-162

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