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Positive selection of murine CD11b + cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator |
Mouse splenocytes before separation | CD11b-depleted splenocytes |
CD11b MicroBeads, human and mouseFigure 2Positive selection of murine CD11b + cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator | CD11b MicroBeads, human and mouseFigure 2Positive selection of murine CD11b + cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator |
Isolated CD11b + mouse cells | |
CD11b MicroBeads, human and mouseFigure 2Positive selection of murine CD11b + cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator |
Our lab has developed different mouse models to study the efficacy of therapeutic candidates against HIV. We have developed a mouse line that is transgenic for some human cell receptors that make mouse cells infectable with HIV. In order to study HIV infection in the brain we isolate microglia from mouse brains and infect the cells in vitro.
To prepare bone marrow-derived macrophages, we isolate CD11b+ bone marrow cells from mice to differentiate into macrophages in culture. To do this, we use CD11b microbeads as a reliable, affordable, easy, and quick way to isolate CD11b+ cells from total bone marrow cells.
Positive selection of murine CD11b + cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator |
Mouse splenocytes before separation | CD11b-depleted splenocytes |
CD11b MicroBeads, human and mouseFigure 2Positive selection of murine CD11b + cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator | CD11b MicroBeads, human and mouseFigure 2Positive selection of murine CD11b + cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator |
Isolated CD11b + mouse cells | |
CD11b MicroBeads, human and mouseFigure 2Positive selection of murine CD11b + cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator |
Our lab has developed different mouse models to study the efficacy of therapeutic candidates against HIV. We have developed a mouse line that is transgenic for some human cell receptors that make mouse cells infectable with HIV. In order to study HIV infection in the brain we isolate microglia from mouse brains and infect the cells in vitro.
To prepare bone marrow-derived macrophages, we isolate CD11b+ bone marrow cells from mice to differentiate into macrophages in culture. To do this, we use CD11b microbeads as a reliable, affordable, easy, and quick way to isolate CD11b+ cells from total bone marrow cells.
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